模板法制备大孔硅胶微球及其在高效液相色谱蛋白质分离中的应用

发布时间：2018-12-06 08:56

【摘要】：以弱阳离子交换聚合物微球(WCX)为模板、N-三甲氧基硅基丙基-N,N,N-三甲基氯化铵(TMSPTMA)为结构导向剂、四乙氧基硅烷(TEOS)为硅胶前驱体,在三乙醇胺弱碱催化作用下,水解缩合形成有机聚合物与二氧化硅复合微球,将此复合微球煅烧后得到大孔二氧化硅微球。探索了不同反应条件对二氧化硅微球的形貌、表面结构和分散性的影响;当TMSPTMA、TEOS与三乙醇胺的体积比为1∶2∶2时可以得到孔径在50~150 nm之间、粒径在2μm左右的硅胶微球。对所制备的大孔硅胶微球表面进行C18(十八烷基二甲基氯硅烷)键合修饰,然后将键合的填料装填到50 mm×4.6 mm的色谱柱中,考察了其对常见的几种标准蛋白质和市售大豆分离蛋白质的分离效果,结果显示这种填料在高效液相色谱蛋白质分离中具有一定的潜力。
[Abstract]:Using weakly cation exchange polymer microsphere (WCX) as template, N-trimethoxysilylpropyl-N _ (trimethylammonium chloride) (TMSPTMA) as structure guide agent, tetraethoxysilane (TEOS) as silica gel precursor, tetraethoxy silane as precursor, tetraethoxysilane as silica gel precursor. Under the catalysis of triethanolamine weak base, the organic polymer and silica composite microspheres were synthesized by hydrolysis and condensation. After calcining the composite microspheres, the macroporous silica microspheres were obtained. The effects of different reaction conditions on the morphology, surface structure and dispersion of silica microspheres were investigated. When the volume ratio of TMSPTMA,TEOS to triethanolamine was 1:2:2, silica microspheres with pore size of 50 ~ 150 nm and diameter of about 2 渭 m could be obtained. C18 (octadecyl dimethyl chlorosilane) bonding modification was performed on the surface of the prepared macroporous silica gel microspheres, and then the bonded filler was filled into a 50 mm 脳 4. 6 mm column. The results showed that this kind of packing has some potential in the separation of protein by high performance liquid chromatography (HPLC).
【作者单位】：上海理工大学理学院化学系;
【基金】：上海市科委项目(14440502300) 上海理工大学沪江基金(D15011)~~
【分类号】：O652.63;TQ127.2;TQ936

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