HPLC同时测定新疆维药芹菜根提取物中3种黄酮类成分的含量

发布时间：2018-12-07 11:09

【摘要】：目的建立同时测定新疆维药芹菜根提取物中3种黄酮类成分含量的HPLC方法。方法 poroshell EC-C_(18)色谱柱(4.6 mm×150 mm,2.7μm),流动相为乙腈-0.1%磷酸(20∶80),流速0.6 ml·min~(-1),检测波长340 nm,柱温28℃,进样量5μl。结果芹菜苷、3'-甲氧基芹菜苷、芹菜素-7-O-β-D-吡喃葡萄糖苷的质量分别在0.046~0.274μg(r=0.9999),0.025~0.153μg(r=0.9999),0.010~0.062μg(r=0.9998)内与峰面积线性关系良好,加样回收率均值分别为97.81%,97.02%,99.85%,RSD为1.81%,1.50%,1.61%。结论表明所建立的方法简便、快速、准确、重现性好,可用于新疆维药芹菜根提取物中黄酮类成分的含量测定。
[Abstract]:Objective to establish a HPLC method for simultaneous determination of three flavonoids in celery root extract from Xinjiang. Methods the mobile phase of poroshell EC-C_ (18) column (4.6 mm 脳 150 mm,2.7 渭 m),) was acetonitrile-0.1% phosphoric acid (20:80). The flow rate was 0.6 ml min~ (-1). Results the mass of apigenin, 3-methoxyapigenin and apigen-7-O- 尾 -D-pyranoside were 0.046 ~ 0.274 渭 g (r ~ (0.9999), 0.025 ~ (0.153) 渭 g (r ~ (0.9999), respectively. The linear relationship between 0.010 and 0.062 渭 g (r 0.9998) and peak area was good. The average recovery rate was 97.81%, 97.02% and 99.85%, respectively. The RSD was 1.81% and 1.50% respectively. The results showed that the method was simple, rapid, accurate and reproducible, and could be used for the determination of flavonoids in celery root extract from Xinjiang.
【作者单位】：新疆医科大学中医学院;新疆名医名方与特色方剂学重点实验室;
【基金】：国家自然科学基金(No.81460590) 新疆医科大学研究生创业启动基金项目(No.CXCY071)
【分类号】：R29;O657.72

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