有机双光子线粒体内活性小分子荧光探针研究进展
发布时间：2018-12-15 15:53
【摘要】：线粒体在细胞的能量代谢中发挥着关键作用,其内部环境的微小变化会影响细胞的正常生命活动。同时线粒体内许多活性小分子在细胞的许多生理过程中也起着关键作用。因此,可视化监测线粒体自身及内部微环境的变化对于生命现象的研究和疾病的诊断与治疗具有非常重要的意义。荧光分析法因具有操作简便、灵敏度高、选择性好、实时检测以及损伤小等优点而得到了广泛的研究和应用。双光子荧光探针技术相对于单光子荧光技术具有长波吸收短波发射、高度的三维空间选择性、大的穿透深度、避免荧光漂白和光致毒以及降低组织自发荧光干扰等特点,在生命科学领域具有广阔的应用前景。该文介绍了有机双光子吸收基本原理以及有机双光子线粒体内活性小分子荧光探针的研究现状,同时对有机线粒体内活性小分子荧光探针今后的发展趋势进行了展望。
[Abstract]:Mitochondria play a key role in the energy metabolism of cells. At the same time, many active small molecules in mitochondria also play a key role in many physiological processes of cells. Therefore, it is very important for the study of life phenomena and the diagnosis and treatment of diseases to monitor the changes of mitochondria itself and internal microenvironment. Fluorescence analysis has been widely studied and applied because of its advantages of simple operation, high sensitivity, good selectivity, real-time detection and small damage. Compared with single photon fluorescence technique, two-photon fluorescence probe has the advantages of long wave absorption shortwave emission, high three-dimensional selectivity, large penetration depth, avoiding fluorescent bleaching and phototoxicity, and reducing tissue autofluorescence interference. It has a broad application prospect in the field of life science. In this paper, the basic principle of organic two-photon absorption and the research status of active small molecule fluorescence probes in organic two-photon mitochondria are introduced. The development trend of active small molecule fluorescence probes in organic mitochondria is also prospected.
【作者单位】： 周口师范学院化学化工学院;
【基金】：河南省高等学校重点科研项目计划资助(15A430055) 周口师范学院校本项目资助(ZKNUB115201)
【分类号】：O657.3
[Abstract]:Mitochondria play a key role in the energy metabolism of cells. At the same time, many active small molecules in mitochondria also play a key role in many physiological processes of cells. Therefore, it is very important for the study of life phenomena and the diagnosis and treatment of diseases to monitor the changes of mitochondria itself and internal microenvironment. Fluorescence analysis has been widely studied and applied because of its advantages of simple operation, high sensitivity, good selectivity, real-time detection and small damage. Compared with single photon fluorescence technique, two-photon fluorescence probe has the advantages of long wave absorption shortwave emission, high three-dimensional selectivity, large penetration depth, avoiding fluorescent bleaching and phototoxicity, and reducing tissue autofluorescence interference. It has a broad application prospect in the field of life science. In this paper, the basic principle of organic two-photon absorption and the research status of active small molecule fluorescence probes in organic two-photon mitochondria are introduced. The development trend of active small molecule fluorescence probes in organic mitochondria is also prospected.
【作者单位】： 周口师范学院化学化工学院;
【基金】：河南省高等学校重点科研项目计划资助(15A430055) 周口师范学院校本项目资助(ZKNUB115201)
【分类号】：O657.3
【相似文献】
相关期刊论文 前10条
1 王姗姗;;小分子荧光探针在硫醇检测中的最新研究进展[J];科技信息;2010年23期
2 向雨秘;龙少波;朱R，
本文编号：2380914
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2380914.html
教材专著
