岭南特色饮片醋马钱子炮制前后HPLC指纹图谱研究
发布时间：2019-01-06 13:20
【摘要】：目的:通过对广东传统炮制醋马钱子的研究,建立醋马钱子的HPLC指纹图谱,并探讨炮制前、中、后指纹图谱的变化。方法:采用HPLC法,色谱柱:Synergi 4u Polar-RP 80A色谱柱(250 mm×4.6 mm,5μm);流动相:A为水-乙酸-三乙胺(100∶0.2∶0.2),B为乙腈(梯度洗脱);流速:0.8 mL/min;检测波长:254 nm;柱温:25℃;进样量:6μL。结果:醋马钱子炮制品及其生品各相似度均大于0.97,相对保留时间的重现性好,炮制品RSD≤0.10%,生品RSD≤0.18%;炮制品和生品共有峰均达16个。结论:建立了醋马钱子的HPLC指纹图谱,并比较了醋马钱子与其生品和中间品之间指纹图谱的差异,研究结果对醋马钱子的炮制机理提供了参考依据。
[Abstract]:Objective: to establish the HPLC fingerprint of semen strychni vinegar by traditional processing in Guangdong province, and to explore the change of fingerprint before, during and after processing. Methods: HPLC method was used. The chromatographic column was Synergi 4u Polar-RP 80A column (250 mm 脳 4.6 mm,5 渭 m); mobile phase: a was water-acetic acid-triethylamine (100: 0.2: 0.2), B) acetonitrile (gradient elution); Flow rate: 0. 8 mL/min; detection wavelength: 254 nm; column temperature: 25 鈩，
本文编号：2402839
[Abstract]:Objective: to establish the HPLC fingerprint of semen strychni vinegar by traditional processing in Guangdong province, and to explore the change of fingerprint before, during and after processing. Methods: HPLC method was used. The chromatographic column was Synergi 4u Polar-RP 80A column (250 mm 脳 4.6 mm,5 渭 m); mobile phase: a was water-acetic acid-triethylamine (100: 0.2: 0.2), B) acetonitrile (gradient elution); Flow rate: 0. 8 mL/min; detection wavelength: 254 nm; column temperature: 25 鈩，
本文编号：2402839
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