慕萨莱思酒中原花青素的HPLC测定方法
发布时间：2019-01-10 13:29
【摘要】：以矢车菊素、儿茶素、表儿茶素、表儿茶素没食子酸酯为标准品,采用岛津LC-20A高效液相色谱仪、二极管阵列检测器,建立慕萨莱思酒中原花青素含量的HPLC测定方法。优化的色谱条件为:Inertsil DOS-3,5μm 4.6 mm×250 mm色谱柱;流动相A为甲醇,流动相B为超纯水,流动相比例:流动相A为35%,流动相B为65%;流速1.0 m L/min;进样量10μL;柱温38℃。采用建立的HPLC方法对慕萨莱思酒进行分析,出峰效果良好;标准曲线正常,回收率为82%~93%。本方法操作简单、准确度高、测定时间短,可用于慕萨莱思酒中原花青素的测定。
[Abstract]:A HPLC method for the determination of proanthocyanidins in Musalez wine was established by using LC-20A high performance liquid chromatograph and diode array detector with cortisone catechin and epigallocatechin gallate as standard. The optimized chromatographic conditions were as follows: Inertsil DOS-3,5 渭 m 4.6 mm 脳 250 mm column, mobile phase A was methanol, mobile phase B was ultrapure water, mobile phase ratio was: mobile phase A was 35g, mobile phase B was 65%, flow rate was 1.0 mL / min, sample volume was 10 渭 L. The column temperature is 38 鈩，
本文编号：2406372
[Abstract]:A HPLC method for the determination of proanthocyanidins in Musalez wine was established by using LC-20A high performance liquid chromatograph and diode array detector with cortisone catechin and epigallocatechin gallate as standard. The optimized chromatographic conditions were as follows: Inertsil DOS-3,5 渭 m 4.6 mm 脳 250 mm column, mobile phase A was methanol, mobile phase B was ultrapure water, mobile phase ratio was: mobile phase A was 35g, mobile phase B was 65%, flow rate was 1.0 mL / min, sample volume was 10 渭 L. The column temperature is 38 鈩，
本文编号：2406372
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