超高效液相色谱-串联质谱法测定动物源食品中螺虫乙酯及其代谢物残留

发布时间：2019-02-16 16:12

【摘要】：建立了一种超高效液相色谱-串联质谱(UPLC-MS/MS)测定动物源食品中的螺虫乙酯及其4种主要代谢物残留的方法。样品采用改进的Qu ECh ERS方法提取和净化,采用电喷雾正离子(ESI+)模式电离,多反应离子监测模式定性分析,基质匹配标准曲线外标法定量。螺虫乙酯及其4种主要代谢物的定量限分别为0.050、0.30、0.030、0.10、0.15μg/kg;在(0.005~0.200)mg/L浓度范围内,螺虫乙酯及其4种主要代谢物的线性相关系数均大于0.999;在0.005、0.050、0.200 mg/kg添加水平范围内,牛乳、猪肉和鸡蛋样品的平均添加回收率在81.6%~107%之间,相对标准偏差在2.1%~9.4%之间(n=5)。该方法简单、快速,灵敏度及准确度高,能够满足动物源食品中螺虫乙酯及其代谢物残留量的检测要求。
[Abstract]:An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of spirochthyl ethyl ester and its four major metabolites in animal food was established. The samples were extracted and purified by improved Qu ECh ERS method, ionization by electrospray positive ion (ESI) mode, qualitative analysis by multi-reaction ion monitoring mode, and quantitative analysis by external standard curve method of matrix matching. The quantitative limit of ethyl screw and its four main metabolites were 0.050 ~ 0.30 ~ 0.30 ~ 0.30 ~ 0.100.10 ~ (-1) 渭 g 路kg ~ (-1) 路kg ~ (-1); In the concentration range of (0.005 ~ 0.200) mg/L, the linear correlation coefficients of ethyl spirochthyl and its four main metabolites were all greater than 0.999; The average recovery of milk, pork and egg samples was between 81.6% and 107%, and the relative standard deviation was between 2.1% and 9.4% (n ~ (5) in the range of 0.005 ~ 0.050 ~ 0.200 mg/kg. The method is simple, rapid, sensitive and accurate. It can be used to determine the residue of ethyl snail and its metabolites in animal food.
【作者单位】：中国热带农业科学院分析测试中心海南省热带果蔬产品质量安全重点实验室;琼台师范学院数理系;
【基金】：海南省应用技术研发与示范推广专项(ZDXM2015044) 海南自然科学基金面上项目(20163078) 海南省高等学校科学研究项目(Hnky2016ZD-14)
【分类号】：O657.63;TS207.53

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