基于氨基化碳量子点荧光增强测定氨苄青霉素
发布时间：2019-02-28 12:57
【摘要】：采用水热法制备碳量子点,通过碳量子点与氨水反应使其表面氨基化,氨苄青霉素与氨基化碳量子点作用后,使其荧光显著增强,由此建立测定药物中氨苄青霉素的荧光光度法。在比色管中依次加入0.016mol·L~(-1)氨基化碳量子点溶液1.00mL和样品溶液0.45mL,pH 7.0三酸缓冲溶液1mL,用水定容至5mL,室温下反应30min后,在激发波长340nm、发射波长433nm处测量溶液相对荧光强度(F/F0,F、F0分别为加入与不加入氨基化碳量子点时体系的荧光强度)。氨苄青霉素质量浓度在2.0×10~(-6)~9.0×10~(-5) mol·L~(-1)内与体系的相对荧光强度呈线性关系,检出限(3S/N)为1.2×10~(-6) mol·L~(-1)。加标回收率为99.0%~109%,测定值的相对标准偏差(n=5)小于2.0%。
[Abstract]:The carbon quantum dots were prepared by hydrothermal method. The surface of carbon quantum dots was ammonified by the reaction of carbon quantum dots with ammonia, and the fluorescence of ampicillin and aminated carbon quantum dots was enhanced significantly by the reaction of ampicillin and ammoniated carbon quantum dots. A fluorometric method for the determination of ampicillin in drugs was established. After adding 0.016mol 路L ~ (- 1) amino carbon quantum dot solution 1.00mL and sample solution 0.45 mL, pH 7.0 triacid buffer solution 1 mL, constant volume of 5 mL with water, the excitation wavelength is 340 nm after the reaction of 30min at room temperature. The relative fluorescence intensity of the solution was measured at the emission wavelength 433nm (F _ (?) F _ (0), F _ (0) is the fluorescence intensity of the system with and without the addition of amino carbon quantum dots). The relative fluorescence intensity of ampicillin was linear in the range of 2.0 脳 10 ~ (- 6) ~ 9.0 脳 10 ~ (- 5) mol 路L ~ (- 1) and the detection limit (3S/N) was 1.2 脳 10 ~ (- 6) mol 路L ~ (- 1). The recovery was 99.0% ~ 109%, and the relative standard deviation (n = 5) was less than 2.0%.
【作者单位】： 忻州师范学院化学系;
【基金】：忻州师范学院重点建设学科项目(XK201303);忻州师范学院大学生科技创新项目(2015);忻州师范学院化学化工实践创新基地基金资助
【分类号】：O657.3;TQ460.72
，
本文编号：2431816
[Abstract]:The carbon quantum dots were prepared by hydrothermal method. The surface of carbon quantum dots was ammonified by the reaction of carbon quantum dots with ammonia, and the fluorescence of ampicillin and aminated carbon quantum dots was enhanced significantly by the reaction of ampicillin and ammoniated carbon quantum dots. A fluorometric method for the determination of ampicillin in drugs was established. After adding 0.016mol 路L ~ (- 1) amino carbon quantum dot solution 1.00mL and sample solution 0.45 mL, pH 7.0 triacid buffer solution 1 mL, constant volume of 5 mL with water, the excitation wavelength is 340 nm after the reaction of 30min at room temperature. The relative fluorescence intensity of the solution was measured at the emission wavelength 433nm (F _ (?) F _ (0), F _ (0) is the fluorescence intensity of the system with and without the addition of amino carbon quantum dots). The relative fluorescence intensity of ampicillin was linear in the range of 2.0 脳 10 ~ (- 6) ~ 9.0 脳 10 ~ (- 5) mol 路L ~ (- 1) and the detection limit (3S/N) was 1.2 脳 10 ~ (- 6) mol 路L ~ (- 1). The recovery was 99.0% ~ 109%, and the relative standard deviation (n = 5) was less than 2.0%.
【作者单位】： 忻州师范学院化学系;
【基金】：忻州师范学院重点建设学科项目(XK201303);忻州师范学院大学生科技创新项目(2015);忻州师范学院化学化工实践创新基地基金资助
【分类号】：O657.3;TQ460.72
，
本文编号：2431816
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