白屈菜红碱分子印迹磁性聚合物微球的制备与性能研究

发布时间：2019-03-02 08:03

【摘要】：白屈菜红碱(CHE)是一种生物活性丰富的生物碱,可抗癌、杀菌,并具有良好的灭螺特性,但在植物体内含量较少、分离困难,因此它的高效分离一直备受人们的关注。本文合成了一种新型的核-壳-壳结构的分子印迹磁性聚合物微球,研究了该微球对白屈菜红碱的吸附动力学与热力学,并进行了白屈菜红碱的高效分离研究。本文还深入探讨了白屈菜红碱与螺类功能蛋白——钥孔戚血蓝蛋白(KLH)的相互作用机理,为白屈菜红碱作为天然灭螺药物的开发提供了有益的参考。具体研究内容如下:(1)以白屈菜红碱为模板,四乙烯基吡啶为功能单体,Fe3O4为磁性基质,采用自由基聚合法合成了白屈菜红碱分子印迹磁性聚合物微球(CHE-MMIPs),并对其进行了形貌表征。结果表明,该微球具有较窄的粒径分布(约240nm),良好的磁响应度(40emu/g),较好的单分散性。(2)考察了CHE-MMIPs的吸附效率。结果表明,CHE-MMIPs最大的吸附容量为12mg/g(6 h),吸附过程符合Langmuir等温吸附模型及准二级动力学方程,属单层吸附。(3)利用上述实验纯化的CHE,采用荧光光谱与分子对接技术研究了CHE对KLH的荧光猝灭机制。结果表明,CHE可以显著猝灭KLH的荧光,其猝灭机制为静态猝灭。在293K、298K、303K时,CHE与KLH的结合常数分别为4.72×105、5.06×105、1.01×106L/mol。体系的热力学参数ΔG298K、ΔH298K、ΔS298K的值分别为 32.54 kJ/mol、 58.78 kJ/mol、和 88.05 J/mol·K。在结合过程中主要的作用力为范德华力和氢键。采用分子对接技术获得了CHE与KLH结合的具体位置、位点数及结合亲和力的大小。发现KLH中的Cu位点并不是CHE与KLH的潜在结合位点。
[Abstract]:(CHE) is a kind of alkaloid with rich biological activity, it can be anticancer, bactericidal and has good snail killing characteristics. However, it has little content in plants and is difficult to be separated. Therefore, its high efficiency separation has been paid close attention by people all the time. In this paper, a novel core-shell structure molecularly imprinted magnetic polymer microspheres was synthesized. The adsorption kinetics and thermodynamics of the microspheres for chordythrine were studied, and the high efficiency separation of alchlor alkaloids was studied. The mechanism of interaction between chordythrine and keyhole hemocyanin (KLH), a functional protein of snail, was also discussed in this paper, which provided a useful reference for the development of chordythrine as a natural snail killing drug. The main contents are as follows: (1) the molecular imprinted magnetic polymer microspheres (CHE-MMIPs) were synthesized by free radical polymerization using tartarine as template, tetravinylpyridine as functional monomer and Fe3O4 as magnetic matrix. Its morphology was characterized. The results showed that the microspheres had narrow particle size distribution (about 240nm), good magnetic responsivity (40emu/g) and good monodispersity. (2) the adsorption efficiency of CHE-MMIPs was investigated. The results show that the maximum adsorption capacity of CHE-MMIPs is 12mg/g (6-h), adsorption process accords with Langmuir isotherm adsorption model and quasi-second order kinetic equation, and belongs to monolayer adsorption. (3) the CHE, purified from the above experiments belongs to monolayer adsorption. The fluorescence quenching mechanism of CHE on KLH was studied by fluorescence spectroscopy and molecular docking technique. The results showed that CHE could significantly quench the fluorescence of KLH, and its quenching mechanism was static quenching. At 293K, 298K and 303K, the binding constants of CHE and KLH were 4.72 脳 105,5.06 脳 105,1.01 脳 106L mol. The thermodynamic parameters 螖 G298K, 螖 H298K and 螖 S298K are 32.54 kJ/mol, / mol 58.78 kJ/mol, and 88.05 J / mol 路K. The main forces in the bonding process are van der Waals force and hydrogen bond. The binding position, the number of sites and the binding affinity of CHE and KLH were obtained by molecular docking technique. It was found that the Cu site in KLH was not a potential binding site between CHE and KLH.
【学位授予单位】：湖南理工学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O631.3

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