安宫牛黄丸HPLC指纹图谱及其多成分化学模式分析
发布时间：2019-03-09 17:31
【摘要】：目的建立安宫牛黄丸(ANP)HPLC指纹图谱,测定其中6种有效成分的量并进行化学模式分析。方法采用HPLC法,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,检测波长为230 nm,对4个不同厂家生产的12批ANP样品进行测定,并采用中药色谱指纹图谱相似度评价软件进行相似度分析。对主要有效成分栀子苷、京尼平苷酸、盐酸小檗碱、黄芩苷、汉黄芩苷、汉黄芩素进行了定量测定。并采用化学模式识别的方法对不同厂家产品进行分析。结果 4个不同厂家生产的12批ANP的指纹图谱相似度均在0.9以上。对6种有效成分定量测定结果发现不同厂家产品仍存在一定差异,尤其以黄芩所含成分差别较大,说明ANP中黄芩原料药材差异较大,其中C厂家产品中黄芩苷、汉黄芩苷及汉黄芩素的量较其他厂家更为稳定,并且其量普遍高于其他厂家。通过主成分分析中的F综合评分,进一步评价不同厂家样品质量的优劣。结果发现,C厂家产品在综合排名中排名较靠前,其结果与其定量测定结果基本吻合。结论指纹图谱结合有效成分的化学模式分析能更全面地反映ANP的质量和优劣,所建立的方法对进一步合理控制ANP的质量提供了实验依据。
[Abstract]:Objective to establish the (ANP) HPLC fingerprint of Angong Niuhuang Pill, determine the contents of 6 active components and carry out chemical model analysis. Methods gradient elution was carried out with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phase by HPLC method. The detection wavelength was 230nm, for the determination of 12 batches of ANP samples produced by four different manufacturers. The similarity analysis was carried out by the similarity evaluation software of chromatographic fingerprint of traditional Chinese medicine. The main effective components of geniposide, berberine hydrochloride, baicalin and baicalin were determined quantitatively. The chemical pattern recognition method was used to analyze the products of different manufacturers. Results the similarity of 12 batches of ANP fingerprints from 4 different factories was above 0.9. The results of quantitative determination of six kinds of active components showed that there were still some differences among the products from different manufacturers, especially the components contained in Scutellaria baicalensis, which indicated that the raw materials of Scutellaria baicalensis in ANP were different greatly, among which baicalin was found in the products of factory C. Baicalin and baicalin were more stable than other manufacturers, and generally higher than other manufacturers. The F score of principal component analysis was used to evaluate the quality of samples from different factories. The results showed that the products of C manufacturers ranked higher in the overall ranking, and the results were basically consistent with the quantitative results. Conclusion fingerprint analysis combined with chemical model analysis of active components can reflect the quality and quality of ANP more comprehensively. The established method provides experimental basis for further rational control of ANP quality. [WT5HZ] conclusion\?
【作者单位】： 中国药科大学天然药物活性组分与药效国家重点实验室;南京中医药大学附属中西医结合医院;江苏省中医药研究院中药组分与微生态研究中心;
【基金】：江苏省“333高层次人才培养工程”项目(BRA2016506)
【分类号】：O657.72;R286.0
[Abstract]:Objective to establish the (ANP) HPLC fingerprint of Angong Niuhuang Pill, determine the contents of 6 active components and carry out chemical model analysis. Methods gradient elution was carried out with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phase by HPLC method. The detection wavelength was 230nm, for the determination of 12 batches of ANP samples produced by four different manufacturers. The similarity analysis was carried out by the similarity evaluation software of chromatographic fingerprint of traditional Chinese medicine. The main effective components of geniposide, berberine hydrochloride, baicalin and baicalin were determined quantitatively. The chemical pattern recognition method was used to analyze the products of different manufacturers. Results the similarity of 12 batches of ANP fingerprints from 4 different factories was above 0.9. The results of quantitative determination of six kinds of active components showed that there were still some differences among the products from different manufacturers, especially the components contained in Scutellaria baicalensis, which indicated that the raw materials of Scutellaria baicalensis in ANP were different greatly, among which baicalin was found in the products of factory C. Baicalin and baicalin were more stable than other manufacturers, and generally higher than other manufacturers. The F score of principal component analysis was used to evaluate the quality of samples from different factories. The results showed that the products of C manufacturers ranked higher in the overall ranking, and the results were basically consistent with the quantitative results. Conclusion fingerprint analysis combined with chemical model analysis of active components can reflect the quality and quality of ANP more comprehensively. The established method provides experimental basis for further rational control of ANP quality. [WT5HZ] conclusion\?
【作者单位】： 中国药科大学天然药物活性组分与药效国家重点实验室;南京中医药大学附属中西医结合医院;江苏省中医药研究院中药组分与微生态研究中心;
【基金】：江苏省“333高层次人才培养工程”项目(BRA2016506)
【分类号】：O657.72;R286.0
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