一测多评法同时测定补肾清利颗粒中8种成分
发布时间：2019-04-07 20:18
【摘要】：目的建立一测多评(QAMS)法同时测定补肾清利颗粒(BQG)中8种成分京尼平苷酸(GA)、绿原酸、栀子苷、滨蒿内酯、丹酚酸B(SAB)、黄芩苷、槲皮素7-O-α-L-鼠李糖苷(QR)及黄芩素的方法,验证该法在BQG中应用的可行性和技术适应性。方法以8种成分为研究对象,利用QAMS 3种校正方法计算各待测成分与内参物滨蒿内酯的相对校正因子(fk/s),推算待测组分量;对回归方程和3种校正方法计算值与外标法实测值进行误差和相关性评价。结果 8种目标成分在一定范围内线性关系良好(r0.999 9),滨蒿内酯与GA、绿原酸、栀子苷、SAB、黄芩苷、QR及黄芩素的fk/s分别是0.562、0.818、0.627、0.877、0.935、1.113、2.339,且在不同条件下重现性良好;6批样品中各成分量分别为GA 1.633~1.736 mg/g、绿原酸1.777~1.950 mg/g、栀子苷6.017~6.422 mg/g、滨蒿内酯1.765~1.851 mg/g、SAB 2.326~2.522 mg/g、黄芩苷19.476~21.329 mg/g、QR 3.077~3.265 mg/g及黄芩素0.161~0.186 mg/g,且计算所得量与实测量测得结果无显著差异。结论 QAMS法为BQG提供了一个快捷可行的多指标同步质量评价模式。
[Abstract]:Objective to establish a multi-evaluation (QAMS) method for simultaneous determination of geniposide (GA), chlorogenic acid, Gardeniposide, Artemisia lactone, Salvianolic acid B (SAB), baicalin in Bushenqingli granule (BQG). The method of quercetin 7 O-伪-L-rhamnoside (QR) and baicalin was used to verify the feasibility and technical adaptability of this method in BQG. Methods the relative correction factors (fk/s) of each component and the internal reference of Artemisia lactone were calculated by QAMS three correction methods and the components of the components to be measured were calculated. The error and correlation between the calculated values of regression equation and three kinds of correction methods and the measured values by external standard method were evaluated. Results there was a good linear relationship among the eight target constituents (r 0.999 9). The fk/s of Artemisia lactone and GA, chlorogenic acid, geniposide, SAB, baicalin, QR and baicalin were 0.562, 0.818, 0.627, 0.877,0.935, 1.113, 2.339, respectively. And the reproducibility is good under different conditions. The components of 6 batches of samples were GA 1.633 / 1.736 mg/g, chlorogenic acid 1.777 / 1.950 mg/g, gardenoside 6.017 / 6.422 mg/g, artemisia lactone 1.765 / 1.851 mg/g,SAB 2.326 / 2.522 mg/g,. Baicalin 19.476 ~ 21.329 mg/g,QR 3.077 ~ 3.265 mg/g and baicalin 0.161 ~ 0.186 mg/g,. There was no significant difference between calculated and measured results. Conclusion QAMS provides a fast and feasible multi-index synchronous quality evaluation model for BQG.
【作者单位】： 南京中医药大学药学院;江苏省中药高效给药系统工程技术研究中心;南京市中药微丸产业化工程技术研究中心;
【基金】：江苏省科技厅产学研前瞻性联合项目(BY2013008-01) 江苏高校优势学科建设工程资助项目(2015)
【分类号】：O657.72;R286.0
[Abstract]:Objective to establish a multi-evaluation (QAMS) method for simultaneous determination of geniposide (GA), chlorogenic acid, Gardeniposide, Artemisia lactone, Salvianolic acid B (SAB), baicalin in Bushenqingli granule (BQG). The method of quercetin 7 O-伪-L-rhamnoside (QR) and baicalin was used to verify the feasibility and technical adaptability of this method in BQG. Methods the relative correction factors (fk/s) of each component and the internal reference of Artemisia lactone were calculated by QAMS three correction methods and the components of the components to be measured were calculated. The error and correlation between the calculated values of regression equation and three kinds of correction methods and the measured values by external standard method were evaluated. Results there was a good linear relationship among the eight target constituents (r 0.999 9). The fk/s of Artemisia lactone and GA, chlorogenic acid, geniposide, SAB, baicalin, QR and baicalin were 0.562, 0.818, 0.627, 0.877,0.935, 1.113, 2.339, respectively. And the reproducibility is good under different conditions. The components of 6 batches of samples were GA 1.633 / 1.736 mg/g, chlorogenic acid 1.777 / 1.950 mg/g, gardenoside 6.017 / 6.422 mg/g, artemisia lactone 1.765 / 1.851 mg/g,SAB 2.326 / 2.522 mg/g,. Baicalin 19.476 ~ 21.329 mg/g,QR 3.077 ~ 3.265 mg/g and baicalin 0.161 ~ 0.186 mg/g,. There was no significant difference between calculated and measured results. Conclusion QAMS provides a fast and feasible multi-index synchronous quality evaluation model for BQG.
【作者单位】： 南京中医药大学药学院;江苏省中药高效给药系统工程技术研究中心;南京市中药微丸产业化工程技术研究中心;
【基金】：江苏省科技厅产学研前瞻性联合项目(BY2013008-01) 江苏高校优势学科建设工程资助项目(2015)
【分类号】：O657.72;R286.0
【相似文献】
相关期刊论文 前10条
1 杨建华;郭萍;戴琴;承强;李晖;;基于粉末X射线衍射技术的黄芩素晶体结构解析[J];四川大学学报(工程科学版);2012年06期
2 郭蒙;王淑敏;王佳丽;李丹;高庆宇;李丽敏;;光谱和电化学方法研究黄芩素与核酸相互作用的机理[J];应用化学;2014年01期
3 侯珏卓;陈U，
本文编号：2454391
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2454391.html
教材专著
