pH对DNA折纸纳米结构折叠与溶液稳定性的影响
发布时间：2019-04-08 12:12
【摘要】：研究了不同pH环境对2种使用M13链作为骨架链的常见DNA折纸折叠与稳定性的影响,获得了其在pH=4~11这一广泛范围内的耐受性数据.首先观察了在不同pH缓冲液中的三角形DNA折纸自组装折叠行为,发现其合适pH范围为6~9,过高或过低的pH都不能退火形成折纸结构.另一方面,又研究了在标准pH缓冲液中预先制备的DNA折纸,对不同pH环境的耐受性.研究发现,在pH值为5~10的溶液环境中,DNA折纸均保持一定稳定.比较以上2种研究方法获得的结果,可以发现,相比在不同pH缓冲液中从头退火制备,已形成的DNA折纸对酸碱有较好耐受性.另外,研究也发现,长方形折纸和三角形折纸稳定性相似,都能在pH=5~10的环境中保持至少12 h的稳定性.据此,还提出了pH对折纸稳定性的可能影响机理,认为其对氢键的破坏及对DNA链造成的断裂共同导致了DNA折纸结构的破碎.
[Abstract]:The effects of different pH environments on folding and stability of two kinds of common DNA origami with M13 chains as frame chains were studied, and their tolerability data in a wide range of pH=4~11 were obtained. First, the self-assembly and folding behavior of triangular DNA origami in different pH buffer was observed. It was found that the suitable pH range was 6? 9, and too high or too low pH could not anneal to form origami structure. On the other hand, the tolerance of DNA origami prepared in standard pH buffer to different pH environments was studied. It was found that the DNA origami kept certain stability in the solution environment of pH 5? 10. Comparing the results obtained by the above two methods, it can be found that the formed DNA origami is more tolerant to acid and base than that prepared in different pH buffers by ab initio annealing. In addition, it is found that the stability of rectangular origami is similar to that of triangular origami, and the stability of both rectangular origami and triangular origami can be kept at least 12 h in the environment of pH=5~10. Based on this, the possible mechanism of the influence of pH on the stability of origami paper is put forward. It is considered that the destruction of hydrogen bond and the fracture of DNA chain result in the fragmentation of DNA origami structure.
【作者单位】： 中国科学院上海应用物理研究所;中国科学院大学;
【基金】：国家重点基础研究发展计划(973计划,项目号2013CB932803) 国家自然科学基金(基金号21473236,31371015) 中国科学院青年创新促进会资助项目
【分类号】：O629.7
，
本文编号：2454563
[Abstract]:The effects of different pH environments on folding and stability of two kinds of common DNA origami with M13 chains as frame chains were studied, and their tolerability data in a wide range of pH=4~11 were obtained. First, the self-assembly and folding behavior of triangular DNA origami in different pH buffer was observed. It was found that the suitable pH range was 6? 9, and too high or too low pH could not anneal to form origami structure. On the other hand, the tolerance of DNA origami prepared in standard pH buffer to different pH environments was studied. It was found that the DNA origami kept certain stability in the solution environment of pH 5? 10. Comparing the results obtained by the above two methods, it can be found that the formed DNA origami is more tolerant to acid and base than that prepared in different pH buffers by ab initio annealing. In addition, it is found that the stability of rectangular origami is similar to that of triangular origami, and the stability of both rectangular origami and triangular origami can be kept at least 12 h in the environment of pH=5~10. Based on this, the possible mechanism of the influence of pH on the stability of origami paper is put forward. It is considered that the destruction of hydrogen bond and the fracture of DNA chain result in the fragmentation of DNA origami structure.
【作者单位】： 中国科学院上海应用物理研究所;中国科学院大学;
【基金】：国家重点基础研究发展计划(973计划,项目号2013CB932803) 国家自然科学基金(基金号21473236,31371015) 中国科学院青年创新促进会资助项目
【分类号】：O629.7
，
本文编号：2454563
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