高效毛细管区带电泳法同时检测3个产地沙棘果粉中的活性物质

发布时间：2019-04-16 12:15

【摘要】：利用高效毛细管区带电泳法,对3个产地沙棘果粉中的芦丁、山柰酚、异鼠李素、槲皮素4种活性物质进行同时分离测定。考察不同电泳条件对分离效率的影响,得出最佳电泳条件:缓冲溶液20 mmol/L Na_2B_4O_7-H_3BO_3(质量浓度为1.5 mg/mL的β-环糊精),pH 9.55,检测波长370 nm,分离电压25 kV,进样时间5 s。在该条件下,芦丁、山柰酚、异鼠李素、槲皮素4种活性物质在11 min内得到分离,线性范围分别为0.01~0.51、0.05~0.93、0.02~0.65、0.03~0.81 mg/mL,相关系数在0.997 1~0.999 1之间,检出限分别为5.05×10~(-5)、2.10×10~(-5)、3.75×10~(-5)、1.31×10~(-5) mg/mL(RSN=3),各物质线性关系良好。日内及日间精密度范围为0.08%~4.72%,平均回收率在95.51%~104.66%之间,相对标准偏差不大于3.92%(n=3)。在优化条件下,对4种活性物质标准品的混合物进行测定,并用相同方法对3个产地沙棘果粉中活性物质的提取液进行检测。结果表明:不同产地沙棘中,4种被检测化合物的含量各不相同。沙棘中被测4种化合物总量最高的产自新疆乌鲁木齐,最低的产自甘肃武威,而槲皮素含量以产自山西吕梁的最高,山柰酚和异鼠李素以产自新疆乌鲁木齐最高。本方法快速简便、准确可靠,可用于沙棘果粉中多种活性物质的同时检测。
[Abstract]:Four active substances (rutin, kaempferol, isorhamnetin and quercetin) in Hippophae rhamnoides powder from three producing areas were separated and determined by high performance capillary zone electrophoresis (HPCE). The effects of different electrophoretic conditions on the separation efficiency were investigated, and the optimum electrophoretic conditions were obtained as follows: buffer solution 20 mmol/L Na_2B_4O_7-H_3BO_3 (mass concentration 1.5 mg/mL 尾-cyclodextrin), pH 9.55, p-cyclodextrin), pH 9.55, mass concentration 1.5 mg/mL), Detection wavelength 370 nm, separation voltage 25 kV, injection time 5 s. Under these conditions, four active substances, rutin, kaempferol, isorhamnetin and quercetin, were separated in 11 min. The linear ranges were 0.01 ~ 0.51, 0.05 ~ 0.93, 0.02 ~ 0.65, 0.03 ~ 0.81 mg/mL, respectively. The correlation coefficients were 0.997 脳 10 ~ (- 5) mg/mL (RSN=3), and the detection limits were 5.05 脳 10 ~ (- 5), 2.10 脳 10 ~ (- 5), 3.75 脳 10 ~ (- 5) and 1.31 脳 10 ~ (- 5) respectively. The intra-day and inter-day precision ranges are 0.08% and 4.72% respectively. The average recovery is between 95.51% and 104.66%. The relative standard deviation is less than 3.92% (n = 3). Under the optimized conditions, the mixture of four kinds of active substances was determined, and the extract of active substances from three producing areas of seabuckthorn fruit powder was detected by the same method. The results showed that the contents of four compounds in Hippophae rhamnoides from different producing areas were different. The highest total amount of four compounds in Hippophae rhamnoides was from Urumqi, Xinjiang, and the lowest was from Wuwei, Gansu Province. The quercetin content was the highest in Lv Liang, Shanxi, and the highest in kaempferol and isorhamnetin in Xinjiang Urumqi. The method is rapid, simple, accurate and reliable, and can be used for simultaneous determination of various active substances in seabuckthorn fruit powder.
【作者单位】：山西师范大学化学与材料科学学院;山西大同大学化学与环境工程学院;
【基金】：国家自然科学基金面上项目(21375083)
【分类号】：O658.9;TS278

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