分散液液微萃取-分光光度法测定食用菌中镉的含量
发布时间：2019-05-14 00:17
【摘要】：取经清洗、粉碎并烘干的样品0.500 0g,用硝酸5mL及过氧化氢3mL,按程序升温模式微波消解。消解液于沸水浴中蒸发至约1mL,用水定容至50mL。取此溶液5.00mL依次加入0.2g·L~(-1) 5-Br-PADAP溶液2.0mL,氨性缓冲溶液(pH 9.0)3.0mL及100g·L~(-1) Triton X-114溶液3.0mL,加水定容至25mL,摇匀,使Cd~(2+)生成络合物,10min后加入辛醇1.0mL,涡旋混合1min,离心5min,吸出下层溶液,取出上层红色有机层,用乙醇定容至3mL,于540nm处用1cm比色皿测得其吸光度。镉的质量浓度在10.00mg·L~(-1)以内与吸光度呈线性关系,检出限(3s)为0.05mg·L~(-1)。加标回收率为93.3%~103%,测定值的相对标准偏差(n=6)小于5.0%。
[Abstract]:The sample, which was cleaned, crushed and dried, was digested with nitric acid 5mL and hydrogen peroxide 3 mL, and digested by microwave in temperature programmed mode. The hydrolysate evaporated to about 1 mL in boiling water bath and fixed volume to 50 mL with water. The solution 5.00mL was added to 0.2 g 路L ~ (- 1) 5-Br-PADAP solution 2.0 mL, ammonia buffer solution (pH 9.0) 3.0mL and 100g 路L ~ (- 1) Triton X 鈮，
本文编号：2476287
[Abstract]:The sample, which was cleaned, crushed and dried, was digested with nitric acid 5mL and hydrogen peroxide 3 mL, and digested by microwave in temperature programmed mode. The hydrolysate evaporated to about 1 mL in boiling water bath and fixed volume to 50 mL with water. The solution 5.00mL was added to 0.2 g 路L ~ (- 1) 5-Br-PADAP solution 2.0 mL, ammonia buffer solution (pH 9.0) 3.0mL and 100g 路L ~ (- 1) Triton X 鈮，
本文编号：2476287
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