跌打活血散气相色谱指纹图谱

发布时间：2019-05-14 04:34

【摘要】：目的:建立跌打活血散气相色谱指纹图谱,为制剂的质量评价提供参考。方法:建立制剂GC指纹图谱,使用中药色谱指纹图谱相似度评价系统(2008版)计算相似度;采用GC-MS法,通过对照品比对,NIST谱库及文献检索对共有峰进行指认,并与对照药材比对确定其来源。结果:方法学考察显示建立的GC指纹图谱精密度、重复性、稳定性均可满足指纹图谱分析的需要;指纹图谱中呈现20个共有峰,对其中的17个进行了指认;14批样品的指纹图谱相似度评价结果在0.564~0.986,表明不同样品的质量差异较大。结论:建立的GC指纹图谱方法快速、简便,能够全面反映跌打活血散中化学成分信息,可用于跌打活血散质量评价。
[Abstract]:Objective: to establish the fingerprint of Diedahuoxue Powder by gas chromatography (GC), and to provide reference for the quality evaluation of the preparation. Methods: the GC fingerprint of the preparation was established, and the similarity was calculated by using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (version 2008). GC-MS method was used to identify the common peaks by comparison of reference materials, NIST spectrum database and literature retrieval, and the source was determined by comparison with the control medicinal materials. Results: the methodological investigation showed that the precision, reproducibility and stability of the established GC fingerprint could meet the needs of fingerprint analysis, and there were 20 common peaks in the fingerprint, 17 of which were identified. The similarity evaluation results of fingerprints of 14 batches of samples were 0.564 鈮，

本文编号：2476439