六味地黄丸三维同步荧光光谱特性的研究

发布时间：2019-05-17 07:47

【摘要】：应用英国Edinburgh公司FLS920P荧光光谱仪,实验测定了六味地黄丸组分中熟地黄、山茱萸、山药、泽泻、茯苓、牡丹皮等六味中药的三维同步荧光光谱,并提取荧光特征参数,发现六味中药均为荧光物质,谱图线形各不相同,具有指纹特性,且与药理相吻合。进一步,实验测定六味地黄丸的标准水煎剂及非标准水煎剂的三维同步荧光光谱,发现两者差异明显,可用于区分不同组方的中成药水煎剂。实验与理论均表明:将三维荧光光谱分析方法与同步荧光光谱分析方法相结合,可以进一步提高荧光光谱技术的灵敏度和选择性,在多组分复杂混合物的荧光光谱分析中有明显的优势。因此,本文的工作为建立完整信息中药材指纹图谱提供了一种方便快捷可靠的方法,也可为中成药的品质鉴别和成分分析提供帮助。
[Abstract]:The three-dimensional synchronous fluorescence spectra of Rehmannia glutinosa, Cornus officinalis, Chinese yam, alisma orientalis, Poria umbellus and peony peel in Liuwei Dihuang pills were determined by FLS920P fluorescence spectrometer of Edinburgh Company, and the fluorescence characteristic parameters were extracted. It was found that all the six traditional Chinese medicines were fluorescent substances, the spectra were different, and they had fingerprint characteristics, which were consistent with pharmacology. Furthermore, the three-dimensional synchronous fluorescence spectra of standard decoction and non-standard decoction of Liuwei Dihuang Pill were determined, and it was found that the difference between them was obvious and could be used to distinguish the decoction of Chinese patent medicine with different prescriptions. The experimental and theoretical results show that the sensitivity and selectivity of the fluorescence spectroscopy can be further improved by combining the three-dimensional fluorescence spectrum analysis method with the synchronous fluorescence spectrum analysis method. It has obvious advantages in fluorescence spectrum analysis of multicomponent complex mixtures. Therefore, the work of this paper provides a convenient, fast and reliable method for the establishment of complete information fingerprint of Chinese medicinal materials, and can also provide help for the quality identification and composition analysis of proprietary Chinese medicines.
【作者单位】：江南大学理学院;江南大学物联网学院;江苏省轻工光电工程技术研究中心;上海市中西医结合医院;
【基金】：国家自然科学基金项目(61178032,61378037) 中央高校基本科研业务费专项资金项目(JUSRP51517)资助
【分类号】：R286.0;O657.3

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