高效液相色谱法同时测定血浆中吲哚美辛和布洛芬的浓度

发布时间：2019-05-21 20:28

【摘要】：建立了同时测定血浆中吲哚美辛和布洛芬两种非甾体抗炎药的HPLC测定方法。样品采用乙腈沉淀蛋白提取后离心,上清液用氮气吹干流动相复溶,以乙腈:20 mmol/L乙酸铵水溶液(甲酸调节p H为3.5)=85:15(V/V)进行洗脱,在ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱上分离,流速为1 m L/min,检测波长220 nm,采用内标法定量,吲哚美辛和布洛芬互为内标。结果表明吲哚美辛和布洛芬在0.1~50μg/m L范围内呈良好的线性关系,吲哚美辛和布洛芬的检出限(S/N=3)分别为1.3 ng和2.0 ng。回收率在86.3%~102.0%之间,日内精密度RSD鄒3.4%。经方法学验证,该方法适用于血浆样品中吲哚美辛和布洛芬的测定,且方法专属性好、简便快捷。
[Abstract]:A HPLC method for the simultaneous determination of indomethacin and ibuprofen in plasma was established. The sample was extracted with acetonitrile precipitated protein and centrifuged. The liquid was dissolved again with nitrogen blowing dry mobile phase and eluted with acetonitrile: 20 mmol/L ammonium acetate aqueous solution (adjusted pH 3.5 by formic acid) = 85:15 (V 鈮，

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