有机分子荧光探针成像检测线粒体内ROS的研究进展

发布时间：2019-05-22 00:31

【摘要】：线粒体是细胞的"能量工厂",它利用氧气进行氧化磷酸化产生三磷酸腺苷(ATP),为细胞及生命体提供能量.同时,伴随着呼吸链中电子的泄漏,多种活性氧物种(ROS)在线粒体内快速产生.线粒体ROS在维持氧化还原平衡、参与调控细胞的增殖、分化、凋亡等行为方面发挥重要作用.当ROS水平超过机体抗氧化防御能力时,会导致疾病的发生.因此,发展准确检测线粒体ROS的方法,对深入探究ROS的细胞功能调控及相关疾病的发生发展意义重大.由于ROS存在浓度低、寿命短、反应活性高等特点,对其进行精准检测是化学、生物学及医学领域的一大挑战.荧光成像技术具有时空分辨率高、生物相容性好、灵敏度高等显著优势,成为实时检测细胞及活体内ROS的有力工具.近年来,相继发展了诸多荧光探针,实现了线粒体ROS的成像分析.本文着重总结与评述了近年来发展靶向线粒体、荧光可视化多种ROS有机分子探针工作的研究进展,并在构建新型线粒体荧光探针、进一步利用荧光成像方法深入剖析线粒体ROS的细胞学功能等方面进行了探讨与展望.
[Abstract]:Mitochondria are the "energy factories" of cells, which use oxygen for oxidative phosphorylation to produce adenosine triphosphate (ATP), to provide energy for cells and organisms. At the same time, with the leakage of electrons in the respiratory chain, a variety of reactive oxygen species (ROS) are produced rapidly in mitochondria. Mitochondrial ROS plays an important role in maintaining redox balance and regulating cell proliferation, differentiation, apoptosis and other behaviors. When the level of ROS exceeds the antioxidant defense ability of the body, it will lead to the occurrence of disease. Therefore, the development of accurate detection of mitochondrial ROS is of great significance to further explore the cellular function regulation of ROS and the occurrence and development of related diseases. Because of the characteristics of low concentration, short life and high reaction activity, accurate detection of ROS is a major challenge in the fields of chemistry, biology and medicine. Fluorescence imaging technology has the advantages of high temporal and spatial resolution, good biocompatibility and high sensitivity. It has become a powerful tool for real-time detection of ROS in cells and in vivo. In recent years, many fluorescence probes have been developed to realize the imaging analysis of mitochondrial ROS. In this paper, the research progress of targeting and visualization of ROS organic molecular probes in recent years is reviewed, and a new type of mtDNA fluorescent probes is constructed. Furthermore, the cellular function of mitochondrial ROS was analyzed by fluorescence imaging.
【作者单位】：山东师范大学化学化工与材料科学学院分子与纳米探针教育部重点实验室;
【基金】：国家重点基础研究发展计划(编号:2013CB933800) 国家自然科学基金(编号:21390411,21535004,21475079,21675105) 长江学者奖励计划教育部“创新团队发展计划”(编号:IRT_16R46)资助项目
【分类号】：O657.3;Q26

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