HPLC法同时测定灯盏细辛注射液中10种成分

发布时间：2019-05-23 11:04

【摘要】：目的建立HPLC法同时测定灯盏细辛注射液(灯盏细辛)中5-咖啡酰基奎宁酸、绿原酸、4-咖啡酰基奎宁酸、咖啡酸、1,3-二咖啡酸酰奎宁酸、野黄芩苷、异绿原酸B、3,5-二咖啡酰基奎宁酸、灯盏花甲素、4,5-二咖啡酰基奎宁酸的含有量。方法该药物水提液的分析采用Inertsil屮ODS-3色谱柱(250 mm×4.6 mm,5μm);流动相0.4%磷酸-乙腈,梯度洗脱;体积流量0.9 m L/min;柱温30℃;检测波长327 nm。结果 10种成分在各自范围内线性关系良好(r≥0.999 0),平均加样回收率94.11%~100.64%,RSD 1.12%~2.77%。结论该方法简便准确,重复性好,可用于灯盏细辛注射液的质量控制。
[Abstract]:Objective to establish a HPLC method for the simultaneous determination of 5-caffeine quinic acid, chlorogenic acid, 4-coffee-quinic acid, caffeine, 1, 3-dicicarboxyquinic acid, baicalin and isochlorogenic acid in Erigeron breviscapus injection (Erigeron breviscapus). 3, the content of 5-di-coffee acyl quinic acid, breviscapine A, 4, 5-di-coffee acyl quinic acid. Methods the water extract of the drug was analyzed by Inertsil ODS-3 column (250 mm 脳 4.6 mm, 5 渭 m), the mobile phase was 0.4% phosphate-acetonitrile, gradient eluting, the volume flow rate was 0.9 mL / min, the column temperature was 30 鈩，

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