基于寡核苷酸链的汞离子荧光生物传感器
发布时间：2019-06-07 11:28
【摘要】：基于G-四链体结构和卟啉类化合物N-甲基卟啉二丙酸IX(NMM)结合产生强烈的荧光,利用T-Hg(Ⅱ)-T错配对汞离子(Hg~(2+))的特异性识别,建立了一种简单、灵敏、高效的Hg~(2+)检测新方法。在富含鸟嘌呤(G)寡核苷酸链中,引入了大量胸腺嘧啶(T)。在没有Hg~(2+)存在时,可以自发形成G-四链体结构,与NMM结合产生强烈的荧光;在Hg~(2+)存在时,可与另一条富含T序列的互补链通过T-Hg(Ⅱ)-T特异性结合,形成双链DNA分子,从而导致G-四链体结构不能产生。优化后最佳实验条件为:缓冲溶液的pH=6.7,20 mmol/L KCl,2.5μmol/L NMM,反应时间为2 h。在优化条件下,体系的荧光强度变化值与Hg~(2+)浓度呈现良好的线性关系,线性范围为50~1000 nmol/L,检出限为22.8 nmol/L(3σ)。此生物荧光传感器对Hg~(2+)具有良好的选择性。实际水样中Hg~(2+)的加标回收率为106.1%~107.8%,可以满足实际水样品中Hg~(2+)的检测要求。
[Abstract]:Based on the strong fluorescence produced by the binding of G-tetrachain structure and N-methylporphyrin dipropionic acid IX (NMM) to produce strong fluorescence, a simple method was established by using the specific recognition of mercury ion (Hg~ (2) by T-Hg (II)-T mismatch. A new sensitive and efficient method for Hg~ (2) detection. A large amount of thymidine (T). Was introduced into guanine-rich (G) oligodeoxynucleotides. In the absence of Hg~ (2), the G-quadruchain structure can be formed spontaneously and bind to NMM to produce strong fluorescence. In the presence of Hg~ (2), it can bind specifically to another complementary chain rich in T sequence through T-Hg (II)-T to form double-stranded DNA molecule, which leads to the inability of G-quadruchain structure. The optimum experimental conditions were as follows: the reaction time of pH=6.7,20 mmol/L KCl, 2.5 渭 mol / L NMM, was 2 h. Under the optimized conditions, the fluorescence intensity of the system has a good linear relationship with the concentration of Hg~ (2), and the linear range is 50 鈮，
本文编号：2494759
[Abstract]:Based on the strong fluorescence produced by the binding of G-tetrachain structure and N-methylporphyrin dipropionic acid IX (NMM) to produce strong fluorescence, a simple method was established by using the specific recognition of mercury ion (Hg~ (2) by T-Hg (II)-T mismatch. A new sensitive and efficient method for Hg~ (2) detection. A large amount of thymidine (T). Was introduced into guanine-rich (G) oligodeoxynucleotides. In the absence of Hg~ (2), the G-quadruchain structure can be formed spontaneously and bind to NMM to produce strong fluorescence. In the presence of Hg~ (2), it can bind specifically to another complementary chain rich in T sequence through T-Hg (II)-T to form double-stranded DNA molecule, which leads to the inability of G-quadruchain structure. The optimum experimental conditions were as follows: the reaction time of pH=6.7,20 mmol/L KCl, 2.5 渭 mol / L NMM, was 2 h. Under the optimized conditions, the fluorescence intensity of the system has a good linear relationship with the concentration of Hg~ (2), and the linear range is 50 鈮，
本文编号：2494759
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