高效液相色谱法分析株洲市中药代煎质量

发布时间：2019-06-22 14:55

【摘要】：目的:以经典方剂四物汤为例,分析株洲市中药代煎质量。方法:随机选取株洲市区23家能够提供中药代煎服务的药房,对同一药房的同批饮片进行实验室规范煎煮(以下简称"自煎")和代煎。建立一种高效液相色谱法同时测定四物汤中5-羟甲基糠醛、芍药苷、阿魏酸和藁本内酯4种活性成分的含量。将23家药房作为整体,通过独立样本t检验,考察同一活性成分在自煎和代煎条件下含量上的差异性。采用逼近于理想值的排序法(TOPSIS)对单一药房两种煎煮方式进行综合评分。结果:TOPSIS分析显示,23家药房中仅有2家代煎优于自煎,占总量的8.70%。在整体水平上,5-羟甲基糠醛和芍药苷在自煎、代煎结果上没有显著性差异。但阿魏酸和藁本内酯自煎、代煎结果有显著性差异。结论:株洲市中药代煎质量在整体上不如实验室规范煎煮,在个别有效成分溶出率上两种煎煮方式有显著性差异。
[Abstract]:Objective: to analyze the quality of traditional Chinese medicine decoction in Zhuzhou City by taking Siwu decoction as an example. Methods: 23 pharmacies which could provide traditional Chinese medicine decoction service in Zhuzhou city were randomly selected. The same batch of decoction pieces in the same pharmacy were cooked in laboratory standard (hereinafter referred to as "self-decoction") and substitute decoction. A high performance liquid chromatography (HPLC) method for the simultaneous determination of 5-hydroxymethyl furfural, paeoniflorin, ferulic acid and ligustilide in Siwu decoction was established. 23 pharmacies were used as a whole to investigate the difference of the content of the same active ingredient between self-decoction and substitute decoction by independent sample t test. The sorting method (TOPSIS), which approximates the ideal value, is used to evaluate the two decoction methods in a single pharmacy. Results: TOPSIS analysis showed that only 2 of the 23 pharmacies were better than self-decoction, accounting for 8.70% of the total. On the whole level, there was no significant difference between 5-hydroxymethyl furfural and paeoniflorin in the results of self-decoction and substitution. However, there was significant difference between ferulic acid and ligustilide in self-decoction. Conclusion: the quality of traditional Chinese medicine decoction in Zhuzhou City is not as good as that in laboratory standard, and there is significant difference between the two decoction methods in the dissolution rate of individual effective components.
【作者单位】：湖南中医药高等专科学校药学系;
【基金】：湖南省大学研究性学习和创新性实验计划项目(编号:2015-644) 湖南省中医药科研计划项目(编号:2013150)
【分类号】：O657.72;R284.1

【相似文献】