加速溶剂萃取法结合HPLC-ESI-TOF MS测定清肝散结汤中29种化学成分（英文）
发布时间：2019-07-06 13:47
【摘要】：清肝散结汤(QGSJ)是由14味不同中药组成的复方制剂,基于长期的实验观察和临床经验以不同组合成方用于肝癌的治疗。本研究建立了一种基于加速溶剂萃取(ASE)结合高效液相色谱-电喷雾飞行时间质谱(HPLC-ESI-TOF MS)的方法同时测定清肝散结汤中29种化学成分。使用70%乙醇作为提取溶剂,提取温度100℃,静态萃取时间5min,提取2次,以上条件采用正交设计和主成分分析进行优化。HPLC分离采用Kromasil C18色谱柱,梯度洗脱。TOF MS在负离子模式下检测,质量扫描范围m/z100~1 100。结果表明:清肝散结汤中29种成分具有良好的线性关系(r0.994)和日内、日间精密度(RSD5%);提取回收率在96.5%~104.5%之间。该方法快速、可靠,适用于复方中药的定量评价。
[Abstract]:Qinggan Sanjie decoction (QGSJ) is a compound preparation composed of 14 different traditional Chinese medicine. Based on long-term experimental observation and clinical experience, different combinations are used in the treatment of liver cancer. In this study, a method based on accelerated solvent extraction (ASE) combined with high performance liquid chromatography-electrospray time-of-flight mass spectrometry (HPLC-ESI-TOF MS) was established for the simultaneous determination of 29 chemical constituents in Qinggan Sanjie decoction. Using 70% ethanol as extraction solvent, the extraction temperature was 100 鈩，
本文编号：2511061
[Abstract]:Qinggan Sanjie decoction (QGSJ) is a compound preparation composed of 14 different traditional Chinese medicine. Based on long-term experimental observation and clinical experience, different combinations are used in the treatment of liver cancer. In this study, a method based on accelerated solvent extraction (ASE) combined with high performance liquid chromatography-electrospray time-of-flight mass spectrometry (HPLC-ESI-TOF MS) was established for the simultaneous determination of 29 chemical constituents in Qinggan Sanjie decoction. Using 70% ethanol as extraction solvent, the extraction temperature was 100 鈩，
本文编号：2511061
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