HPLC法测定生脉注射液中特征成分莽草酸
发布时间：2019-07-25 13:58
【摘要】：采用液质联用法对生脉注射液中莽草酸成分进行确认,建立高效液相色谱法测定生脉注射液中莽草酸含量。采用液质联用法测定目标峰的分子离子峰m/z:173.08[M-H]-,与莽草酸对照品对比确认目标峰为莽草酸成分;色谱柱采用Synergi Hydro-RP 80A(250 mm×4.6 mm,4μm),以20 mmol/L的KH2PO4(H3PO4调p H至2.5)溶液为流动相,流量为0.6 m L/min,检测波长214 nm;莽草酸在0.02~0.64μg范围内呈良好的线性关系,r=0.999 8,精密度、重复性试验RSD分别为0.15%和1.49%,平均加样回收率为99.2%,RSD=1.56%。该研究首次确认生脉注射液中含有莽草酸成分,且该成分来源于五味子;含量测定方法准确、可靠,可作为生脉注射液中五味子成分质量控制检测方法。
[Abstract]:Shikimic acid in Shengmai injection was confirmed by liquid mass spectrometry, and the content of shikimic acid in Shengmai injection was determined by high performance liquid chromatography (HPLC). The molecular ion peak m/z:173.08 of the target peak was determined by liquid mass spectrometry, and compared with shikimic acid reference, the target peak was confirmed to be shikimic acid, the chromatographic column was Synergi Hydro-RP 80A (250 mm 脳 4.6 mm, 4 渭 m), the mobile phase was 20 mmol/L KH2PO4 (H3PO4 adjusted pH to 2.5) solution, the flow rate was 0.6 mL 路min, and the detection wavelength was 214 nm;. Shikimic acid showed a good linear relationship in the range of 0.02 ~ 0.64 渭 g, r 鈮，
本文编号：2519133
[Abstract]:Shikimic acid in Shengmai injection was confirmed by liquid mass spectrometry, and the content of shikimic acid in Shengmai injection was determined by high performance liquid chromatography (HPLC). The molecular ion peak m/z:173.08 of the target peak was determined by liquid mass spectrometry, and compared with shikimic acid reference, the target peak was confirmed to be shikimic acid, the chromatographic column was Synergi Hydro-RP 80A (250 mm 脳 4.6 mm, 4 渭 m), the mobile phase was 20 mmol/L KH2PO4 (H3PO4 adjusted pH to 2.5) solution, the flow rate was 0.6 mL 路min, and the detection wavelength was 214 nm;. Shikimic acid showed a good linear relationship in the range of 0.02 ~ 0.64 渭 g, r 鈮，
本文编号：2519133
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