聚（2-甲基-2-噁唑啉）/聚（4-乙烯基吡啶）二元聚合物刷对蛋白质的可控吸附研究

发布时间：2024-02-25 14:08

　　控制蛋白质在材料表面的吸附和脱附在生物材料或医学等方面十分重要。在不同的情况下,对蛋白质的吸附和脱附有不同的要求。例如,对于细胞和组织在材料表面的增殖以及蛋白质的富集和分离需要蛋白质的吸附,而在生物技术和生物医学材料领域如生物传感器、含血的材料如人工血管、人造心脏等则需要抑制蛋白质的吸附。因此需要设计一种能够根据需求调控蛋白质在其上的吸附和脱附的表面,这一点可以通过同时具有刺激响应性和抗蛋白质吸附性能的表面实现。本论文发展了一种可以实现蛋白质可控吸附的新方法:在材料表面接枝上抗蛋白吸附聚合物刷(聚(2-甲基-2-噁唑啉)(PMOXA))和刺激响应性聚合物刷(聚(4-乙烯基吡啶)(P4VP))。论文分为两个部分。(1)分别通过2-甲基-2-噁唑啉(MOXA)的阳离子开环聚合(CROP)和4-乙烯基吡啶(4VP)的可逆加成-断裂链转移(RAFT)聚合,和随后与甲基丙烯酸缩水甘油酯的无规和嵌段共聚制备了聚(2-甲基-2-噁唑啉-r-甲基丙烯酸缩水甘油酯)(PMOXA-r-GMA)刷状共聚物和聚(4-乙烯基吡啶)-b-聚甲基丙烯酸缩水甘油酯)(P4VP-b-PGMA)。最后,将PMOXA-r-...

【文章页数】：116 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Chapter 1 Introduction and Background
    1.1 Polymer Brushes
    1.2 Binary Polymer Brushes
    1.3 Binary Polymer Brushes for Controlled Bio-adhesion
        1.3.1 Oppositely Charged Binary Polymer Brushes
        1.3.2 Hydrophobic Polymer Brushes
        1.3.3 Hydrophilic Polymer Brushes
    1.4 Poly(2-oxazoline)s (POXs)
        1.4.1 Poly(2-oxazoline) (POXs)
        1.4.2 CROP of Oxazolines
        1.4.3 Advantages of POXs
    1.5 Modification of Solid Substrate
        1.5.1 Pattering via "Grafting from" Method
        1.5.2 "Grafting to" Method
    1.6 Objective of This Thesis
    References
Chapter 2 Poly(2-methyl-2-ogazoline) and Poly(4-vinyl pyridine) Based Mixed Brusheswith Switchable Ability Toward Protein Adsorption
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Materials
        2.2.2 Polymers Synthesis
            2.2.2.1 Synthesis of PMOXA-r-GMA
            2.2.2.2 Synthesis of P4VP-b-PGMA
        2.2.3 Surface Modification
        2.2.4 Characterization
            2.2.4.1 Nuclear Magnetic Resonance (NMR)
            2.2.4.2 Fourier Transforms Infrared Spectroscopy (FTIR)
            2.2.4.3 X-ray Photoelectron Spectroscopy (XPS)
            2.2.4.4 Variable Angle Spectroscopic Ellipsometry (VASE)
            2.2.4.5 Atomic Force Microscopy (AFM)
        2.2.5 Switchable Properties
            2.2.5.1 Dry Thickness
            2.2.5.2 Water Contact Angle (WCA)
            2.2.5.3 FITC-BSA Adsorption Assay
            2.2.5.4 Quantification of Protein Adsorption
    2.3 Results and Discussions
        2.3.1 Synthesis of Polymers
        2.3.2 Characterization of Polymer Modified Surface
        2.3.3 Switchable Properties
            2.3.3.1 Thickness
            2.3.3.2 Hydrophilicity
            2.3.3.3 Protein Adsorption Behavior
    2.4 Conclusion
    References
Chapter 3 Reversible Adsorption of Protein on Poly(2-methyl-2-oxazoline) /Poly(4-vinylpyridine) Based Binary Polymer Brushes: Influence of Ionic Strength and P4VP ChainLength
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Materials
        3.2.2 Polymer Synthesis
            3.2.2.1 Synthesis of PMOXA-r-GMA
            3.2.2.2 Synthesis of P4VP-b-PGMA
        3.2.3 Surface Modification
        3.2.4 Characterizations
            3.2.4.1 Nuclear Magnetic Resonance (NMR)
            3.2.4.2 Zeta Potential Assessment
            3.2.4.3 X-ray Photoelectron Spectroscopy (XPS)
            3.2.4.4 Variable Angle Spectroscopic Ellipsometry (VASE)
            3.2.4.5 Atomic Force Microscopy (AFM)
        3.2.5 Switchable Properties
            3.2.5.1 Hydrophilicity
            3.2.5.2 Dry Thickness
            3.2.5.3 Qualitative Analysis of the Protein Adsorption
            3.2.5.4 QCM-D Experiments
    3.3 Results and Discussion
        3.3.1 Synthesis of Polymers
        3.3.2 Characterization of Polymer Modified Surfaces
        3.3.3 Switchable Properties
            3.3.3.1 Hydrophilicity of the Coating
            3.3.3.2 Thickness of the Coating
            3.3.3.3 Zeta Potential Measurements
        3.3.4 Protein Adsorption Studies
    3.4 Conclusion
    References
Chapter 4 Overall Conclusion and Future Work
Acknowledgements
List of Publications



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