接种量对产气肠杆菌同步糖化暗发酵产氢的影响

发布时间：2018-02-25 23:07

  本文关键词： 接种量 产气肠杆菌 同步糖化 生物制氢　出处：《中国沼气》2017年02期 　论文类型：期刊论文

【摘要】：文章以小麦秸秆为原料,研究了不同接种量对产气肠杆菌同步糖化发酵产氢的影响,以期寻求最佳的接种量条件。试验以累积产氢量、产氢速率等指标来分析产气肠杆菌利用小麦秸秆进行同步糖化发酵产氢的潜力及其可行性。结果表明:在以反应液体积为200 mL,底物为5 g小麦秸秆,酶负荷为150 mg·g~(-1)秸秆、初始pH值为6.5,温度为35℃的条件下,接种量为30%时产氢效果最好,此时的累积产气量达到737 mL,累积产氢量达到293mL,最大产氢速率为35.42 mL·h~(-1)L~(-1)。该实验研究为秸秆类生物质同步糖化厌氧暗发酵产氢的进一步研究奠定了基础并提供了科学参考。
[Abstract]:The effect of inoculation amount on hydrogen production by simultaneous saccharification fermentation of Enterobacter aerogenes was studied with wheat straw as raw material in order to find the best inoculation conditions. Hydrogen production rate was used to analyze the potential and feasibility of simultaneous saccharification fermentation with wheat straw by Enterobacter aerogenes. The results showed that when the volume of reaction solution was 200 mL, the substrate was 5 g wheat straw, the enzyme loading was 150 mg 路g ~ (-1). When the initial pH value is 6.5 and the temperature is 35 鈩，

本文编号：1535522