掺锶纳米介孔羟基磷灰石的合成、骨诱导性及作为药物递送载体的研究

发布时间：2018-03-01 19:02

  本文关键词： 纳米HAP 阿伦磷酸钠 体外药物释放 药物递送 骨修复　出处：《河北大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着世界人口老龄化的加剧,骨折成为影响公众健康的重大威胁,骨修复的需求量也随之急剧增加。骨组织工程有望满足日益增加的骨修复需求。纳米级的磷酸钙生物材料因其具有与自然骨的无机成分组成类似的特点,在骨组织修复中的应用研究引起广泛关注。本论文设计合成了四种掺杂不同比例锶的HAP纳米颗粒,通过扫描电子显微镜、透射电子显微镜、X-射线粉末衍射、动态光散射、比表面积测试仪等方法表征了其形貌、尺寸以及组成;通过MTT法、溶血实验、诱导骨矿化等实验评价纳米颗粒的生物相容性和骨诱导性;以纳米HAP为载体,采用紫外、荧光、红外、热重等检测方法,研究了载体对阿伦磷酸钠的吸附、释放行为及释药机制。结果表明:所合成的HAP为棒状的纳米介孔颗粒,长80-100 nm,直径20 nm,介孔孔径2-3 nm;适量的锶掺入HAP纳米颗粒可以提高其生物相容性和骨诱导性;纳米HAP载体通过静电吸附作用,将药物阿仑膦酸钠吸附在载体表面,并以自由扩散的形式持续地从载体表面释放。所合成的四种HAP纳米颗粒中,载体CS3(20%Sr@HAP)纳米颗粒具有较好的载释药能力,载药48 h后,载药量达到0.85 mg(AL)/mg(载体),体外模拟释药15 d,累积释药量达到76%,故CS3是一个理想的递送双磷酸类药物载体。鉴于棒状纳米介孔HAP载体本身所具备的促进成骨细胞分化和矿化的能力,且载体所缓释的阿伦磷酸钠具有抑制破骨细胞的骨吸收能力,我们推测,在治疗骨质疏松过程中,载体与药物可能起到协同治疗的作用,在骨质疏松治疗方面具有潜在的应用前景。
[Abstract]:As the world population aging intensifies, fracture become a major threat to public health, demand for bone repair are sharply increasing. Bone tissue engineering is expected to meet the increasing demand for bone repair. The nano calcium phosphate biomaterials because of its advantages and inorganic composition of natural bone consists of similar characteristics, research and application in bone tissue repair caused widespread concern. Four kinds of HAP nanoparticles doped with different proportion of strontium were designed and synthesized in this paper, by scanning electron microscopy, transmission electron microscopy, X- ray diffraction, dynamic light scattering, specific surface area analyzer were used to characterize the morphology, size and composition; through MTT method, hemolysis test the experimental evaluation of induced bone mineralization, nanoparticle biocompatibility and osteoinductive; nano HAP as the carrier, by UV, fluorescence, infrared, thermal gravimetric detection method of carrier on 闃夸鸡纾烽吀閽犵殑鍚搁檮,閲婃斁琛屼负鍙婇噴鑽�鏈哄垼�

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