光合细菌混合菌群HAU-M1产氢动力学实验研究

发布时间：2018-03-09 06:06

本文选题：光合细菌混合菌群　切入点：混合菌群　出处：《太阳能学报》2016年06期 　论文类型：期刊论文

【摘要】：研究光合细菌混合菌群以葡萄糖为底物,光合产氢过程中的生长和产氢动力学特性,分析混合菌群利用葡萄糖产氢过程中的基质降解规律及代谢产物的生成规律。研究结果表明:光合细菌混合菌群以葡萄糖为底物产氢时,150 mmol/L的葡萄糖添加量是最佳添加浓度,产氢过程存在代谢产酸的过程,产氢高峰期葡萄糖主要代谢产物为乙酸,此时产气速率大;产氢末期葡萄糖主要代谢产物为丁酸,此时产气速率较低。建立基于Monod方程的光合细菌混合菌群产氢过程中的生长动力学模型,模型可较好地描述混合菌群产氢过程中生长延滞期和对数增长期菌体的生长变化规律,最大比生长速率μmax为0.214 h~(-1),饱和常数KS为8.257。建立光合细菌混合菌群产氢过程中的底物消耗动力学模型,模型可较好地描述混合菌群产氢过程中产氢延滞期和产氢高峰期的葡萄糖降解规律,细胞得率系数YX/S为0.352 g/mol,维持系数m为0.85。
[Abstract]:To study the growth and hydrogen-producing kinetic characteristics of photosynthetic bacteria mixed with glucose as substrate during the process of photosynthetic hydrogen production. The law of substrate degradation and the formation of metabolites in the process of hydrogen production by glucose were analyzed. The results showed that the optimum concentration of glucose was the amount of glucose added to the mixed bacteria at 150 mmol/L when glucose was used as the substrate for hydrogen production. The main metabolite of glucose is acetic acid, which is the main metabolite of hydrogen production, and the main metabolite of glucose in the end of hydrogen production is butyric acid, the main metabolite of glucose is butyric acid at the end of hydrogen production. Based on Monod equation, the growth kinetics model of mixed photosynthetic bacteria during hydrogen production was established. The model can describe the growth and variation of bacteria in the growth delay period and logarithmic growth period during the hydrogen production process of the mixed bacteria. The maximum specific growth rate (渭 max) was 0.214 h ~ (-1) and the saturation constant (K _ S) was 8.257. A kinetic model of substrate consumption in the process of hydrogen production by photosynthetic bacteria was established. The model can describe the glucose degradation law of hydrogen production delay period and hydrogen production peak in the process of hydrogen production of mixed bacteria. The cell yield coefficient (YX/S) is 0.352 g / mol, and the maintenance coefficient (m) is 0.85.
【作者单位】：河南农业大学农业部农村可再生能源新材料与装备重点实验室;华北水利水电大学电力学院;
【基金】：国家自然科学基金(50976029;U1504509) 教育部博士点基金(20134105130001)
【分类号】：TQ116.2

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