壳聚糖与量子点及配位金属离子的电沉积技术研究

发布时间：2018-04-03 03:39

本文选题：电沉积技术　切入点：壳聚糖　出处：《武汉理工大学》2015年硕士论文

【摘要】：微电子技术和生物技术是20世纪发展最为迅速的两大技术,进入21世纪人们迫切希望将这两种技术结合起来进行应用。近年来,研究者通过结合微电子技术与生物技术,对一些电刺激响应性生物大分子的电沉积技术开展了研究,其中壳聚糖的电沉积技术研究受到了广泛关注,壳聚糖的电沉积技术具有操作简单、条件温和、时间和空间选择性和可控性等性能特点和优点,被广泛应用于生物传感器、药物控制释放、储能材料、生物燃料电池等领域。本工作开展了壳聚糖与量子点电沉积技术的研究,还开展了壳聚糖与配位金属离子电沉积技术的研究。量子点(QDs)呈现出许多特殊的物理和化学性能,被广泛应用于生物检测、生物成像、生物传感器、荧光图案等领域。本工作中我们开展了壳聚糖与Zn S量子点电沉积技术的研究,首先制备了一种水性ZnS QDs,再利用电沉积技术实现了壳聚糖/ZnS量子点沉积膜的制备,研究了电沉积条件对制备的壳聚糖/ZnS量子点沉积膜荧光强度的影响,然后采用不同形状的电极及保护性涂层的方法进行了壳聚糖/Zn S量子点荧光图案的制备,以及荧光图案的消除与重复性再现。研究结果表明利用电沉积技术能够实现在不同电极(金、银、钛及316L不锈钢)上制备壳聚糖/ZnS量子点沉积膜,且制备的壳聚糖/ZnS量子点沉积膜具有明显的蓝色荧光,并且沉积膜的荧光强度可以通过改变电沉积条件进行控制;此外,利用电沉积技术能够实现在不同类型的电极或基材上制备壳聚糖/ZnS量子点荧光图案,该荧光图案可以消除和重复性再现,该方法在构建光电子器件、荧光涂层和图案、生物电子器件的等领域具有潜在的应用价值。壳聚糖分子可以与配位金属离子通过配位作用生成凝胶,本工作利用金属铜作为阳极,利用电沉积技术实现了壳聚糖/Cu2+水凝胶的制备,经过一系列的实验对壳聚糖/Cu2+水凝胶电沉积机理进行了验证,研究了电沉积条件(沉积时间、沉积电压)对壳聚糖/Cu2+电沉积的影响。研究结果显示采用金属铜作为阳极,利用电沉积技术能够实现壳聚糖/Cu2+水凝胶的制备,制备的壳聚糖/Cu2+水凝胶表面平整、无气泡,呈现了与Cu2+颜色相同的蓝色,壳聚糖/配位金属离子水凝胶的厚度可以通过调节电沉积条件进行控制;该壳聚糖/配位金属离子的电沉积技术既具有常规阴极电沉积技术的时间和空间选择性和可控性,又具有配位金属离子的选择性。因此,该壳聚糖/配位金属离子的电沉积不仅为壳聚糖的电沉积技术提供了一种新型、简单、可控的方法,而且制备的壳聚糖/配位金属离子凝胶在生物电子器件、固定蛋白质、表面涂层及图案化、药物控制释放等领域具有潜在的应用价值。
[Abstract]:Microelectronics and biotechnology are the two most rapidly developing technologies in the 20th century. In the 21st century, people are eager to combine these two technologies for application.In recent years, researchers have carried out research on electrodeposition of some responsive biomolecules by combining microelectronics and biotechnology, among which the research of chitosan electrodeposition has been paid more and more attention.Chitosan electrodeposition technology has been widely used in biosensor, controlled drug release, energy storage materials, biofuel cells and other fields because of its simple operation, mild conditions, time and space selectivity and controllability.In this work, the electrodeposition of chitosan and QDs was carried out, and the electrodeposition of chitosan and coordination metal ions was also studied.Quantum Dots (QDs) show many special physical and chemical properties, which are widely used in the fields of biological detection, biological imaging, biosensor, fluorescence patterns and so on.In this work, we studied the electrodeposition of chitosan and ZnS quantum dots. Firstly, we prepared a kind of water-borne ZnS QDs, and then we realized the preparation of chitosan / ZnS quantum dot films by electrodeposition.The effect of electrodeposition conditions on the fluorescence intensity of the prepared chitosan / ZnS quantum dots was studied. Then the fluorescence patterns of chitosan / ZnS quantum dots were prepared by using different electrodes and protective coatings.And the elimination of fluorescent patterns and repetitive reproduction.The results show that chitosan / ZnS quantum dot films can be deposited on different electrodes (gold, silver, titanium and 316L stainless steel) by electrodeposition.Moreover, the fluorescence intensity of the deposited films can be controlled by changing the electrodeposition conditions. In addition, the fluorescence patterns of chitosan / ZnS quantum dots can be prepared on different types of electrodes or substrates by electrodeposition.The fluorescence pattern can be eliminated and reproduced repeatedly. This method has potential application value in the construction of optoelectronic devices, fluorescent coatings and patterns, bioelectronic devices and so on.Chitosan molecules can coordinate with coordination metal ions to form gels. In this work, the preparation of chitosan / Cu _ 2 hydrogels was realized by electrodeposition using metal copper as anode.The electrodeposition mechanism of chitosan / Cu _ 2 hydrogel was verified by a series of experiments. The effect of electrodeposition conditions (deposition time, deposition voltage) on the electrodeposition of chitosan / Cu _ 2 was studied.The results show that the preparation of chitosan / Cu _ 2 hydrogel can be realized by using metal copper as anode and electrodeposition technology. The prepared chitosan / Cu _ 2 hydrogel has a flat surface and no bubble, showing the same blue color as Cu2.The thickness of chitosan / coordination metal ion hydrogel can be controlled by adjusting the electrodeposition conditions, and the electrodeposition of the chitosan / coordination metal ion has the temporal and spatial selectivity and controllability of conventional cathodic electrodeposition.It also has the selectivity of coordination metal ions.Therefore, the electrodeposition of the chitosan / coordination metal ions not only provides a new, simple and controllable method for the electrodeposition of chitosan, but also makes the chitosan / coordination metal ion gel in bioelectronic devices.The fields of fixed protein, surface coating and patterning, drug controlled release have potential application value.
【学位授予单位】：武汉理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TQ153;O636.1

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