聚羟基丁酸戊酸酯（PHBV）多孔膜的制备及生物相容性研究

发布时间：2018-04-11 03:12

  本文选题：聚羟基丁酸戊酸酯 + 多孔膜　； 参考：《兰州理工大学》2017年硕士论文

【摘要】：石油基高分子材料广泛的应用于各个领域,对人类的生活和国民的经济产生着重要作用,但随着能源材料危机的不断加深以及白色污染的加剧,可持续发展的生物基高分子材料便备受青睐。聚羟基脂肪酸酯(Polyhydroxyalkanoats,PHA)是惟一一个完全由微生物合成的生物降解基的高分子材料。同时PHA相对于其他生物材料(聚乳酸PLA、丁二酸丁二醇共聚物PBS、聚羟基乙酸PGA、淀粉基塑料等)结构多元化,种类繁多,物化性能不尽相同,可以适用于不同条件下的应用要求,PHA已成为生物材料领域最为活跃的研究热点之一。高分子超滤膜已在颗粒脱除、蛋白等物质的分离与纯化、水质净化、污水处理、血液透析和细胞多孔支架等众多领域得到广泛应用。可制备超滤膜的高分子大部分为石油基高分子材料,膜材料依赖与石油能源,而且废弃后不具备生物降解性能,易对环境造成新的污染,因此开发源于可再生资源并具备生物可降解性的新型高分子多孔膜材料日益受到关注,此外具有生物相容性的多孔膜在生物医用领域应用日益收到重视。本文以二甲基乙酰胺(DMAc)作为溶剂,采用相转化法(NIPS)制备聚羟基丁酸戊酸酯(PHBV)平板多孔膜,通过对非溶剂、铸膜液浓度、凝固温度、凝固浴组成的调节、添加剂等,调控成膜过程中的相分离行为,制备出结构可控的PHBV多孔膜。并用生物质聚羟基丁酸戊酸酯多孔膜作为细胞培养的培养基质,培养Hela细胞,观察细胞生长状况,探究聚羟基丁酸戊酸酯多孔膜的生物相容性。研究表明,45℃时PHBV/DMAc/H_2O体系分相比PHBV/DMAc/乙醇体系更快。当凝固浴为纯水时,非溶剂和溶剂交换速率快,发生瞬时分相,而PHBV固化速率较低,多孔膜形貌呈指状孔、海绵孔并存。随着凝固浴中溶剂DMAc的加入,非溶剂和溶剂的交换速率变缓,从而发生延时分相,多孔膜呈均匀的海绵状结构。添加剂中聚乙烯吡咯烷酮PVP对多孔膜的成孔效果最佳,不仅有利于多孔膜的形成,而且下表面形成排列规整的蜂窝状孔,蜂窝状孔内有小孔存在,孔径分布比较均匀。亲水物质PVP的加入,也是多孔膜的水通量得到提高。在浸润温度为80℃时,凝固浴中为纯水时,相分离过程中,热力学与动力学相互作用,使PHBV/PVP多孔膜的成孔效果好,孔隙率和水通量相对较高。用PHBV多孔膜作为细胞培养基质,对Hela细胞进行细胞培养,不同细胞培养基质,Hela细胞的Viability值在90%以上,证明PHBV多孔膜无毒性,细胞可以在上面进行生长,不会对细胞产出影响。并通过DAPI染剂对细胞进行染色,在荧光显微镜下观察到,蜂窝状PHBV多孔膜作为细胞培养基质时,Hela细胞生长、繁殖情况好。因此PHBV多孔膜为生物相容性好,可以应用在生物医学领域。
[Abstract]:Petroleum based polymer materials are widely used in various fields, which play an important role in human life and national economy. However, with the deepening of the crisis of energy materials and the intensification of white pollution,Sustainable development of biological-based polymer materials will be favored.Polyhydroxyalkanoate (PHA) is the only biodegradable polymer material completely synthesized by microorganisms.At the same time, compared with other biomaterials (polylactic acid (PLA), butylene succinate copolymer (PHA), polyglycolic acid (PGA), starch based plastics, etc.), the structure of PHA is various, and its physical and chemical properties are different.PHA, which can be applied to different conditions, has become one of the most active research hotspots in the field of biomaterials.Polymer ultrafiltration membrane has been widely used in many fields, such as particle removal, protein separation and purification, water purification, sewage treatment, hemodialysis and cellular porous scaffolds.Most of the polymers that can be prepared by ultrafiltration membrane are petroleum-based polymer materials. The membrane materials depend on petroleum energy and do not have biodegradability after being abandoned, which is easy to cause new pollution to the environment.Therefore, the development of new polymeric porous membrane materials with biodegradability and renewable resources has attracted more and more attention. In addition, the application of biocompatible porous membrane in biomedical field has been paid more and more attention.In this paper, polyhydroxybutyrate valerate (PHBV) flat porous membranes were prepared by phase inversion method (NIPS) using dimethyl acetamide (DMAC) as solvent. The effects of non-solvent, concentration of casting solution, solidification temperature, composition of coagulation bath, additives, etc.PHBV porous membranes with controllable structure were prepared by regulating the phase separation behavior during the process of membrane formation.Biomass polyhydroxybutyrate valerate porous membrane was used as the culture matrix of cell culture to culture Hela cells, observe the cell growth, and explore the biocompatibility of polyhydroxybutyrate polyvalerate porous membrane.The results show that PHBV/DMAc/H_2O system is faster than PHBV/DMAc/ ethanol system at 45 鈩，

本文编号：1734160