汽化过氧化氢（HPV）发生器的设计与杀菌研究

发布时间：2018-05-20 09:57

  本文选题：汽化过氧化氢发生器 + Polyethylene　； 参考：《江南大学》2017年硕士论文

【摘要】：灭菌在无菌灌装过程中是十分重要的环节,直接影响产品的质量,汽化过氧化氢灭菌技术在国外已作为一种新型绿色灭菌方法用于无菌灌装生产中。而我国汽化过氧化氢灭菌技术还处于起步阶段,其生成设备与国外设备也有很大的差距,因此开发新型的汽化过氧化氢生成设备对提高我国无菌包装技术具有非常重要的现实意义。本文对汽化过氧化氢发生器进行了设计,并通过实验研究了汽化过氧化氢生成工艺参数和操作工况对灭菌效果的影响,主要研究内容如下:1.基于文丘里原理,选择文丘里管射流器作为雾化部件,采用先混合雾化后加热的方法制备汽化过氧化氢。对汽化过氧化氢发生器的主体结构、气液混合及加热系统,流量和温度控制系统各组成部分进行了研究设计。对文丘里管射流器进行了理论计算,并通过雾化试验对射流器进行了验证。对加热器的加热功率进行了计算,选择总功率为6kW的加热器,并采用2 kW和4 kW串联的方法实现对雾化过氧化氢加热和温度控制。2.采用单因素和响应面实验结合法研究了汽化过氧化氢在“未预热-灭菌-不吹干”工况下对Polyethylene Terephthalate(PET)瓶坯内表面枯草芽孢杆菌的杀菌效果。由单因素实验可得,H_2O_2流量和灭菌时间与杀菌效果呈正相关,随溶液流量的增加、灭菌时间的延长,杀菌效果明显增强;而蒸汽温度与灭菌效果呈负相关,随温度的升高,过氧化氢分解加剧使得灭菌效果减弱。在此工况下,由响应面实验得出,H_2O_2流量和灭菌时间的交互作用对灭菌效果的影响较大,根据回归分析结果得出最佳工艺参数为:H_2O_2溶液流量16 m L/min,灭菌时间10 s,蒸汽温度120℃,经验证,测得在此工艺条件下生成的汽化过氧化氢对PET瓶坯中枯草芽孢杆菌的杀菌对数值可达6.85-log(CFU/m L)。3.研究了汽化过氧化氢在工业工况(50℃预热-灭菌-50℃热风干燥)下对PET瓶坯内表面枯草芽孢杆菌的杀菌效果。在单因素的基础上,通过响应面优化实验建立了H_2O_2溶液流量、灭菌时间和蒸汽温度对杀菌对数值的三元二次回归方程,经分析得出,H_2O_2溶液流量和灭菌时间的交互作用对灭菌效果的影响非常显著,此工况的最佳工艺参数为:H_2O_2溶液流量14.24 m L/min,灭菌时间9.05 s,蒸汽温度120℃,该条件下预测灭菌值为5.45-log(CFU/m L),实验灭菌平均值为5.39-log(CFU/mL)。为保证彻底灭菌,提高H_2O_2溶液流量进行试验,结果发现随过氧化氢溶液流量的增大,汽化过氧化氢杀菌效果明显增强。本课题提供了一种汽化过氧化氢发生器的设计方法,通过对汽化过氧化氢灭菌工艺参数和效果的研究,证明了汽化过氧化氢灭菌技术具有效率高,H_2O_2溶液用量少的优势,在无菌灌装中具有很好的应用价值。
[Abstract]:Sterilization is a very important link in the process of aseptic filling, which directly affects the quality of products. Vaporization of hydrogen peroxide sterilization technology has been used as a new green sterilization method in aseptic filling production abroad. However, the vaporization of hydrogen peroxide sterilization technology in China is still in its infancy, and its generating equipment is far from that of foreign countries. Therefore, the development of new vaporized hydrogen peroxide production equipment is of great practical significance to improve the aseptic packaging technology in China. In this paper, the vaporization hydrogen peroxide generator is designed, and the effects of the process parameters and operating conditions on the sterilizing effect are studied experimentally. The main research contents are as follows: 1. Based on Venturi principle, Venturi tube jet was selected as atomizing component to prepare vaporized hydrogen peroxide by mixing atomization and heating. The main structure of vaporized hydrogen peroxide generator, gas-liquid mixing and heating system, flow rate and temperature control system are studied and designed. The theoretical calculation of Venturi tube jet was carried out, and the atomization test was carried out to verify the jet. The heating power of the heater is calculated. The heater with total power of 6kW is selected, and the atomized hydrogen peroxide heating and temperature control. 2 kW and 4 kW in series are adopted. The bactericidal effect of vaporized hydrogen peroxide on Bacillus subtilis (Bacillus subtilis) on the inner surface of Polyethylene Terephthalate (PET) flask was studied by single factor and response surface test. From the single factor experiment, it was found that the volume of H2O2 and sterilizing time were positively correlated with the bactericidal effect. With the increase of the solution flow rate, the sterilization time increased obviously, while the steam temperature was negatively correlated with the sterilizing effect and increased with the increase of the temperature. The decomposition of hydrogen peroxide weakens the sterilizing effect. Under this condition, the interaction between the flow rate and sterilizing time was found to have a great influence on the sterilizing effect. According to the regression analysis, the optimum technological parameters were as follows: flow rate 16ml / min, sterilizing time 10s, steam temperature 120 鈩，

本文编号：1914170