羟基磷灰石表面构建压电陶瓷涂层的生物相容性研究

发布时间：2018-06-04 22:49

  本文选题：羟基磷灰石 + 等离子喷涂　； 参考：《新疆医科大学》2015年硕士论文

【摘要】：目的:评价羟基磷灰石表面构建的压电陶瓷涂层的生物相容性。方法:按照ISO/10993及GB/T16886系列标准,对羟基磷灰石表面构建的压电陶瓷涂层进行检测。1.溶血试验:在稀释抗凝兔血中分别加入羟基磷灰石表面构建的压电陶瓷涂层浸提液、生理盐水与蒸馏水,检测溶血率;2.短期全身毒性试验(经口途径):对大鼠分别灌胃给予羟基磷灰石表面构建的压电陶瓷涂层浸提液与生理盐水,观察动物每周的体重变化,食物利用率;3.热原试验:自新西兰兔耳缘静脉分别注射羟基磷灰石表面构建的压电陶瓷涂层浸提液与生理盐水,观察动物体温变化。4.致敏试验:试验组以羟基磷灰石表面构建的压电陶瓷涂层浸提液与弗氏完全佐剂为供试液,对照组以生理盐水溶液与弗氏完全佐剂为供试液。用最大剂量法进行致敏试验,观察动物的皮肤情况。结果:1.羟基磷灰石表面构建的压电陶瓷涂层浸提液的溶血率为0.86%;2.灌胃一周后,试验组与对照组大鼠体重增长量差异无统计学意义(P0.05),食物利用率分别为16.5%,16.9%,灌胃两周后,试验组与对照组大鼠体重增长量差异无统计学意义(P0.05),食物利用率分别为16.71%,17.1%;3.注射羟基磷灰石表面构建的压电陶瓷涂层浸提液和生理盐水后,试验组兔体温升高0.30℃,试验组兔体温升高总和为0.60℃;4.去除贴敷物24,48,72小时后,试验组与对照组豚鼠的皮肤评分均小于1。结论:羟基磷灰石表面构建的压电陶瓷涂层无溶血作用、无毒性、不致热、无致敏作用,具有良好的生物相容性。
[Abstract]:Objective: to evaluate the biocompatibility of piezoelectric ceramic coating on hydroxyapatite. Methods: according to ISO/10993 and GB/T16886 series standards, the piezoelectric ceramic coating on hydroxyapatite surface was tested. Hemolysis test: dilute anticoagulant rabbit blood by adding hydroxy apatite on the surface of piezoelectric ceramic coating extract, physiological saline and distilled water, to detect the hemolysis rate of 2. 5%. Short-term systemic toxicity test (via oral approach: rats were given hydroxy apatite surface piezoelectric ceramic coating extract and physiological saline respectively, to observe the weekly body weight changes, food utilization rate of 3. 5%. Pyrogen test: piezoelectric ceramic coating extract and physiological saline were injected into the auricular vein of New Zealand rabbit respectively. The changes of body temperature of animals were observed. Sensitization test: the piezoelectric ceramic coating extractant and Freund's complete adjuvant on hydroxyapatite were used as the test solution in the test group, and the physiological saline solution and Freund's complete adjuvant in the control group were used as the test solution. The maximum dose method was used to sensitize animals to observe the skin condition. The result is 1: 1. The hemolysis rate of the piezoelectric ceramic coating solution constructed on hydroxyapatite is 0.86% and 2% respectively. After a week of gavage, there was no significant difference in body weight growth between the experimental group and the control group (P 0.05), but the food utilization rate was 16. 5% and 16. 9% respectively. After two weeks of gavage, there was no significant difference in body weight growth between the experimental group and the control group (P 0. 05), and the food utilization rate was 16. 71% and 17. 1% respectively. The temperature of rabbits in the experimental group was increased by 0.30 鈩，

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