不同钙磷比对激光熔覆生物陶瓷涂层微观结构性能与细胞活性的影响

发布时间：2018-06-30 18:52

本文选题：n(Ca)∶n(P) + 激光熔覆　；参考：《稀有金属》2017年10期

【摘要】：激光熔覆过程中医用钛合金与混合粉末(CaHPO_4·2H_2O+CaCO_3+La_2O_3)因热膨胀系数相差大而容易产生裂纹、气孔等缺陷,故采用梯度设计方法在医用钛合金表面上制备一层具有生物活性的磷酸钙陶瓷涂层。通过金相显微镜(OM),X射线衍射仪(XRD),显微硬度计,甲基噻唑基四唑(MTT)比色法和倒置荧光显微镜对稀土氧化物La_2O_3添加量相同(0.6%)的不同钙磷比激光熔覆磷酸钙陶瓷涂层结合界面、物相、显微硬度、细胞相对活性和细胞生长状态进行分析。试验结果表明:通过OM观察其横截面积可以看出熔覆试样分为基材、合金化层及陶瓷层3个区域,各区域间呈良好的冶金结合。在2θ在32°~33°附近,当n(Ca)∶n(P)(原子比)=1.40~1.45时,其XRD图谱中羟基磷灰石+β-磷酸三钙(HA+β-TCP)的特征峰较高,表明其生成的生物活性相HA+β-TCP的量最多。显微硬度结果表明n(Ca)∶n(P)值越大,涂层平均硬度越高。MTT比色法和倒置荧光显微镜观察结果表明当涂层n(Ca)∶n(P)=1.40时,吸光度值(OD)最大,生物陶瓷涂层上细胞数量最多且生长状态良好,表明该涂层具有最佳的细胞相容性且有利于细胞的稳定增殖与生长。
[Abstract]:During laser cladding, medical titanium alloy and mixed powder (CaHPO42H2OCaCO3) are apt to produce cracks, porosity and other defects due to the large difference in thermal expansion coefficient. Therefore, a bioactive calcium phosphate ceramic coating was prepared on the surface of medical titanium alloy by gradient design method. By means of metallographic microscope (OM) X-ray diffraction (XRD), microhardness meter, methyl thiazolyl tetrazolium (MTT) colorimetry and inverted fluorescence microscope, the interface and phase of different calcium phosphorus ratio laser cladding calcium phosphate ceramic coating with the same amount of rare-earth oxide La _ 2O _ 2O _ 3 (0.6%) were added. Microhardness, cell relative activity and cell growth state were analyzed. The test results show that the cladding sample is divided into three regions by OM observation: substrate alloying layer and ceramic layer. When the ratio of N (Ca): n (P) (atomic ratio) is 1.40 ~ 1.45, the characteristic peak of hydroxyapatite 尾 -tricalcium phosphate (HA 尾 -TCP) is higher in the XRD pattern, indicating that HA 尾 -TCP is the most bioactive phase. The results of microhardness showed that the higher the value of n (Ca): n (P) was, the higher the average hardness of the coating was. MTT colorimetry and inverted fluorescence microscope observation showed that when the coating n (Ca): n (P) was 1.40, the absorbance value (OD) was the highest. The number of cells on the bioceramics coating is the largest and the growth state is good, which indicates that the coating has the best cytocompatibility and is conducive to the stable proliferation and growth of the cells.
【作者单位】：贵州大学材料与冶金学院;贵州省材料结构与强度重点实验室;
【基金】：国家自然科学基金项目(51362004)资助
【分类号】：TQ174.1

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