大豆PDAT基因的鉴定、表达与进化分析

发布时间：2018-04-16 06:47

  本文选题：大豆 + 磷脂　； 参考：《基因组学与应用生物学》2016年03期

【摘要】：PDAT酶催化磷脂和二脂酰甘油反应,生成溶血磷脂和三脂酰甘油,在三脂酰甘油合成过程中扮演着重要作用。为探明大豆PDAT基因的起源与进化关系,本研究利用生物信息学手段剖析了大豆PDAT基因的序列、表达以及进化关系。系统进化关系分析显示,除低等植物外,大豆与其他被子植物PDAT基因被分为4种类群,每一个类群内基因结构与保守基序组成模式均高度保守。基于滑动窗口与位点模型检测选择压力,结果显示大豆PDAT基因均受到功能限制,受控于负选择;但位点-分枝模型发现大豆与其他植物类群Ⅰ和Ⅱ的祖先分支发生了适应性进化。表达分析表明,大豆PDAT基因具有多样化的转录模式,且同源基因对之间存在分化。这些结果为进一步功能分析大豆PDAT基因提供了帮助。
[Abstract]:PDAT enzyme catalyzes the reaction of phospholipids with diacylglycerol to form lysophospholipids and triglycerides, which plays an important role in the synthesis of triglycerides.In order to investigate the relationship between the origin and evolution of soybean PDAT gene, the sequence, expression and evolution of soybean PDAT gene were analyzed by bioinformatics.Phylogenetic analysis showed that the PDAT gene of soybean and other angiosperms was divided into four groups except for lower plants. The gene structure and conserved motif of each group were highly conserved.The selection pressure was detected based on sliding window and locus model. The results showed that soybean PDAT gene was restricted by function and controlled by negative selection.But the local-branching model showed that the progenitor branches of soybean and other plant groups 鈪，

本文编号：1757729