过量表达菠菜SoCYP85A1基因增强烟草的耐盐性

发布时间：2018-06-11 22:25

本文选题：菠菜SoCYPA基因 + 高盐胁迫　；参考：《植物生理学报》2017年03期

【摘要】：本研究以转菠菜SoCYP85A1基因稳定表达的烟草植株为试验材料,检测了其耐盐性。利用NaCl模拟盐胁迫处理野生型和转基因烟草,结果表明:在高盐胁迫下,转基因烟草种子的发芽率显著高于野生型;二者的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均增强,转基因植株的酶活性显著高于野生型;二者的脯氨酸(Pro)含量升高,并且转基因烟草积累量显著高于野生型,而转基因植株的丙二醛(MDA)含量增幅小于野生型;高盐处理后,野生型和转基因烟草的9个胁迫相关的基因表达倍数都增加,同野生型相比,转基因烟草的NtADC1、NtAPX、NtCAT、NtLEA5、NtGST、NtSOD和NtERD10C的表达量显著升高,而NtNCED1和Nt SAMDC基因的表达量变化不显著。本研究结果证实了过量表达SoCYP85A1基因能够通过增强烟草的抗氧化胁迫能力和调节胁迫相关基因的表达来提高烟草的耐盐性,为进一步阐明菠菜SoCYP85A1基因的耐盐机制奠定了理论基础。
[Abstract]:In this study, transgenic tobacco plants with stable expression of SoCYP85A1 gene from spinach were used to test their salt tolerance. Wild type and transgenic tobacco were treated with NaCl simulated salt stress. The results showed that the germination rate of transgenic tobacco seeds was significantly higher than that of wild type under high salt stress. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) were all increased, and the activity of proline in transgenic plants was significantly higher than that of wild type, and the accumulation of transgenic tobacco was significantly higher than that of wild type. However, the increase of MDA content in transgenic plants was lower than that of wild type, and the expression multiple of 9 genes related to stress in wild type and transgenic tobacco increased after high salt treatment, compared with wild type. The expression of NtADC1, NtADC1, NtCATX, NtLEA5, NtGST-NtSOD and NtERD10C in transgenic tobacco was significantly increased, but the expression of NtNCED1 and NtSAMDC was not significantly changed. The results showed that the overexpression of SoCYP85A1 gene could enhance tobacco salt tolerance by enhancing the ability of antioxidant stress and regulating the expression of stress related genes, which laid a theoretical foundation for further elucidating the salt tolerance mechanism of spinach SoCYP85A1 gene.
【作者单位】：青岛农业大学农学与植物保护学院;
【基金】：山东省自然科学基金(ZR2013CQ028) 青岛市应用基础研究计划(16-5-1-54-jch) 青岛农业大学博士基金(663/1111316)~~
【分类号】：Q943.2

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