, and expression analysis of its orthologs in

  本文关键词：番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析，由笔耕文化传播整理发布。

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Abstract  Lysophosphatidic acid acyltransferase (LPAAT) which converts lysophosphatidic acid into phosphatidic acid is a key enzyme in biosynthesis pathway of lipid in plants. In this study, we identified 17 members of the LPAAT gene family from genomic data of G. raimondii-D5 and G. arboreum-A2. Analysis of gene structure, chromosome distribution and phylogenetic evolution of LPAAT genes in diploid Gossypium using bioinformatics approaches showed that these genes can be divided into distinct subfamilies based on the distance of their genetic relationship. Moreover, the gene structures were similar within LPAAT subfamily members. The amino acid sequences encoded by LPAAT family genes contained three conserved motifs, including ΦFPEGTR-G binding site and Φ-NHQS- ΦDΦΦ catalytic site. Phylogenetic analysis of LPAAT gene family demonstrated significant differences in evolution of LPAAT in different species. Finally, expression analysis of G. hirsutum ovules in different stages from RNA-seq and qRT-PCR data indicated that LPAAT gene may play a positive role in oil accumulation. Our studies facilitate understanding of the function of LPAAT gene family in Gossypium and selecting better LPAAT genes for further functional validation.

Received: 05 January 2015     

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Jianjiang Ma,Nuohan Wang,Man Wu, et al. Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum[J]. HEREDITAS(Beijing), 2015, 37(7): 692-701.

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[1] 王彦霞, 刘正杰, 马峙英, 李召虎, 华金平. RNA干涉技术与棉花高油育种. 棉花学报, 2011, 23(2): 178-183.
[2] 刘明, 范君华, 由宝昌, 梅拥军. 海岛棉棉仁蛋白质和脂肪含量分析. 作物品种资源, 1994, (4): 25-28.
[3] 喻树迅, 于霁雯. 棉花作为能源作物的可行性分析. 见: 中华人民共和国农业部编. 中国农村生物质能源国际研讨会暨东盟与中日韩生物质能源论坛论文集. 北京: 中国农业出版社, 2008: 369.
[4] 陈四龙, 黄家权, 雷永, 任小平, 文奇根, 陈玉宁, 姜慧芳, 宴立英, 廖伯寿. 花生溶血磷脂酸酰基转移酶基因的克隆与表达分析. 作物学报, 2012, 38(2): 245-255.
[5] Weselake RJ, Taylor DC, Rahman MH, Shah S, Laroche A, McVetty PBE, Harwood JL. Increasing the flow of carbon into seed oil. Biotechnol Adv , 2009, 27(6): 866-878.
[6] Lewin TM, Wang P, Coleman R A. Analysis of amino acid motifs diagnostic for the sn-glycerol-3-phosphate acyltransferase reaction. Biochemistry , 1999, 38(18): 5764-5771.
[7] Heath RJ, Rock CO. A conserved histidine is essential for glycerolipid acyltransferase catalysis. J Bacteriol , 1998, 180(6): 1425-1430.
[8] Kim HU, Huang AHC. Plastid lysophosphatidyl acyltransferase is essential for embryo development in Arabidopsis. Plant Physiol , 2004, 134(3): 1206-1216.
[9] 尹永泰. 甘蓝型油菜溶血磷脂酸酰基转移酶基因的克隆和表达[学位论文]. 武汉: 华中科技大学, 2011.
[10] 华方静, 刘风珍, 万勇善, 张昆. 花生及其野生种质溶血磷脂酸酰基转移酶基因( LPAAT )的克隆及序列分析. 分子植物育种, 2014, 12(1): 74-79.
[11] Cagliari A, Margis-Pinheiro M, Loss G, Mastroberti AA, de Araujo Mariath JE, Margis R. Identification and expression analysis of castor bean ( Ricinus communis ) genes encoding enzymes from the triacylglycerol biosynthesis pathway. Plant Sci , 2010, 179(5): 499-509.
[12] Knutzon DS, Lardizabal KD, Nelsen JS, Bleibaum JL, Davies HM, Metz JG. Cloning of a coconut endosperm cDNA encoding a 1-acyl-sn-glycerol-3- phosphate acyltransferase that accepts medium-chain-length substrates. Plant Physiol , 1995, 109(3): 999-1006.
[13] Taylor DC, Francis T, Lozinsky S, Hoffman T, Giblin M, Marillia EF. Cloning and characterization of a constitutive lysophosphatidic acid acyltransferase 2 (LPAT2) gene from Tropaeolum majus L. Open Plant Sci J , 2010, 4: 7-17.
[14] Brown AP, Carnaby S, Brough C, Brazier M, Slabas AR. Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene. Biochem J , 2002, 364: 795-805.
[15] Brown AP, Coleman J, Tommey AM, Watson MD, Slabas AR. Isolation and characterisation of a maize cDNA that complements a 1-acyl sn-glycerol-3-phosphate acyltransferase mutant of Escherichia coli and encodes a protein which has similarities to other acyltransferases. Plant Mol Biol , 1994, 26(1): 211-223.
[16] Zou J, Katavic V, Giblin EM, Barton DL, MacKenzie SL, Keller WA, Hu X, Taylor DC. Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell , 1997, 9(6): 909-923.
[17] Maisonneuve S, Bessoule JJ, Lessire R, Delseny M, Roscoe TJ. Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis. Plant Physiol , 2010, 152(2): 670-684.
[18] 张毓婷, 王敏华, 陈家栋, 戎均康, 丁明全. 雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析. 遗传, 2014, 36(9): 921-933.
[19] Li FG, Fan GY, Wang KB, Sun FM, Yuan YL, Song GL, Li Q, Ma ZY, Lu CR, Zou CS, Chen WB, Liang XM, Shang HH, Liu WQ, Shi CC, Xiao GH, Gou CY, Ye WW, Xu X, Zhang XY, Wei HL, Li ZF, Zhang GY, Wang JY, Liu K, Kohel RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX. Genome sequence of the cultivated cotton Gossypium arboreum . Nat Genet , 2014, 46(6): 567-572.
[20] Wang KB, Wang ZW, Li FG, Ye WW, Wang JY, Song GL, Yue Z, Cong L, Shang HH, Zhu SL, Li Q, Yuan YL, Lu CR, Wei HL, Gou CY, Zheng ZQ, Yin Y, Zhang XY, Liu K, Wang B, Song C, Shi N, Kohe RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX. The draft genome of a diploid cotton Gossypium raimondii . Nat Genet , 2012, 44(10): 1098-1103.
[21] Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin DC, Llewellyn D, Showmaker KC, Shu SQ, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu GJ, Lee TH, Li JP, Lin LF, Liu T, Marler BS, Page JT, Roberts AW, Romanel E, Sanders WS, Szadkowski E, Tan X, Tang HB, Xu CM, Wang JP, Wang ZN, Zhang D, Zhang L, Ashrafi H, Bedon F, Bowers JE, Brubaker CL, Chee PW, Das S, Gingle AR, Haigler CH, Harker D, Hoffmann LV, Hovav R, Jones DC, Lemke C, Mansoor S, ur Rahman M, Rainville LN, Rambani A, Reddy UK, Rong JK, Saranga Y, Scheffler BE, Scheffler JA, Stelly DM, Triplett BA, Van Deynze A, Vaslin MF, Waghmare VN, Walford SA, Wright RJ, Zaki EA, Zhang TZ, Dennis ES, Mayer KF, Peterson DG, Rokhsar DS, Wang XY, Schmutz J. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature , 2012, 492(7429): 423-427.
[22] Motamayor JC, Mockaitis K, Schmutz J, Haiminen N, Livingstone D, Cornejo O, Findley SD, Zheng P, Utro F, Royaert S, Saski C, Jenkins J, Podicheti R, Zhao MX, Scheffler BE, Stack JC, Feltus FA, Mustiga GM, Amores F, Phillips W, Marelli JP, May GD, Shapiro H, Ma JX, Bustamante CD, Schnell RJ, Main D, Gilbert D, Parida L, Kuhn DN. The genome sequence of the most widely cultivated cacao type and its use to identify candidate genes regulating pod color. Genome Biol , 2013, 14(6): r53.
[23] Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, FitzGerald LM, Vezzulli S, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT, Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C, Davenport C, Dematte L, Mraz A, Battilana J, Stormo K, Costa F, Tao QZ, Si-Ammour A, Harkins T, Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, Grando S, M, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P, Gutin A, Van de Peer Y, Salamini F, Viola R. A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. PloS One , 2007, 2(12): e1326.
[24] Tanaka T, Antonio BA, Kikuchi S, Matsumoto T, Nagamura Y, Numa H, Sakai H, Wu J, Itoh T, Sasaki T, Aono R, Fujii Y, Habara T, Harada E, Kanno M, Kawahara Y, Kawashima H, Kubooka H, Matsuya A, Nakaoka H, Saichi N, Sanbonmatsu R, Sato Y, Shinso Y, Suzuki M, Takeda JI, Tanino M, Todokoro F, Yamaguchi K, Yamamoto N, Yamasaki C, Imanishi T, Okido T, Tada M, Ikeo K, Tateno Y, Gojobori T, Lin YC, Wei FJ, Hsing YI, Zhao Q, Han B, Kramer MR, McCombie RW, Lonsdale D, O'Donovan CC, Whitfield EJ, Apweiler R, Koyanagi KO, Khurana JP, Raghuvanshi S, Singh NK, Tyagi AK, Haberer G, Fujisawa M, Hosokawa S, Ito Y, Ikawa H, Shibata M, Yamamoto M, Bruskiewich RM, Hoen DR, Bureau TE, Namiki N, Ohyanagi H, Sakai Y, Nobushima S, Sakata K, Barrero RA, Sato Y, Souvorov A, Smith-White B, Tatusova T, An S, An G, OOta S, Fuks G, Messing J, Christie KR, Lieberherr D, Kim H, Zuccolo A, Wing RA, Nobuta K, Green PJ, Lu C, Meyers BC, Chaparro C, Piegu B, Panaud O, Echeverria M. The rice annotation project database (RAP-DB): 2008 update. Nucleic Acids Res , 36(Suppl 1), 2008: D1028-D1033.
[25] Cao JS, Shan DD, Revett T, Li DM, Wu LY, Liu W, Tobin JF, Gimeno RE. Molecular identification of a novel mammalian brain isoform of acyl-CoA: lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2. J Biolog Chem , 2008, 283(27): 19049-19057.
[26] Edgar RC. MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformat , 2004, 5(1): 113.
[27] 王小非, 刘鑫, 苏玲, 孙永江, 张世忠, 郝玉金, 由春香. 番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析. 中国农业科学, 2013, 46(12): 2501-2513.
[28] 涂礼莉, 张献龙, 刘迪秋, 金双侠, 曹景林, 朱龙付, 邓锋林, 谭家福, 张存斌. 棉花纤维发育和体细胞胚发生过程中实时定量 PCR 内对照基因的筛选. 科学通报, 2007, 52(20): 2379-2385.
[29] Livak KJ, Schmittgen TD. Analysis of relative gene ex-pression data using real-time quantitative PCR and the 2 -ΔΔCT method. Methods , 2001, 25(4): 402-408.
[30] 宋俊乔, 孙培均, 张霞, 张献龙, 聂以春, 郭小平, 朱龙付. 棉仁高油分材料筛选及其脂肪酸发育分析. 棉花学报, 2010, 22(4): 291-296.
[31] 陈珊珊, 李然, 俞捷, 汪红志, 张学龙. 永磁低场核磁共振分析仪原理和应用. 生命科学仪器, 2009, 7(10): 49-53.
[32] Hanke C, Wolter FP, Coleman J, Peterek G, Frentzen M. A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia Coli sn -1-acylglycerol- 3-phosphate acyltransferase mutant. Eur J Biochem , 1995, 232(3): 806-810.
[33] Arroyo-Caro JM, Chileh T, Alonso DL, García-Maroto F. Molecular characterization of a lysophosphatidylcholine acyltransferase gene belonging to the MBOAT family in Ricinus communis L. Lipids , 2013, 48(7): 663-674.
[34] Stymne S, Stobart AK. Triacylglycerol biosynthesis. Biochem of Plants , 1987, 9: 175-214.
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  本文关键词：番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析，由笔耕文化传播整理发布。