大豆油脂组分的自然变异分析和相关候选基因鉴定
发布时间：2023-11-02 17:22
大豆(Glycine max L.Merrill)是世界上最重要的油料作物之一,其主要成分是植物蛋白和油脂,被广泛应用于人类的食品消费和加工业。近年来,由于世界上对植物油脂需求的快速增长,因此培育高油大豆品种是育种者最重要的育种目标之一。然而,由于油脂代谢路径相对复杂,致使采用传统遗传改良方法提高大豆种子中的油脂含量十分困难。本研究利用1551份不同来源的大豆种质为材料,采用近红外光谱法(NIRs)和气相色谱(GC)方法,首先评价了大豆种质中的蛋白质,脂肪和脂肪酸(FA)的組成,包括棕榈酸(PA)、硬脂酸(SA)、油酸(OA)、亚油酸(LA)和亚麻酸(LNA),通过2年3点的品质性状的数据分析,明确了中国不同来源大豆种质的主要品质性状的地理分布特点。其次,还系统分析了世界范围内不同国别来源(包括中国、美国、日本和俄罗斯)的633份大豆种质的主要品质性状的变异情况。然后,采用BSA-seq方法,利用两个高低油分含量的大豆种质基因池,鉴定了编码大豆油脂生物合成途径中的关键酶和候选基因,为改良大豆油脂组成的分子育种提供理论依据。其主要研究结果如下:不同地理来源的大豆种质的蛋白质、油脂和脂肪酸...
【文章页数】：162 页
【学位级别】：博士
【文章目录】：
摘要
abstract
LIST OF ABBREVIATION
Chapter1 Introduction
1.1 Uses of soybean
1.1.1 Soybean protein
1.1.2 Soybean oil
1.1.3 Soybean FAs
1.2 Soybean origin and domestication
1.3 Effect of environment on soybean seed compositions
1.3.1 Effects of the environment on soybean protein and oil contents
1.3.2 Effects of the environment on soybean seed FA composition
1.4 Effect of maturity on soybean seed compositions
1.5 Metabolic pathway for oil biosynthesis in plants
1.6 Molecular markers
1.7 Next generation sequencing(NGS)and genotyping techniques
1.8 Significance of research work
1.9 Research objective
Chapter2 Profiling of Seed Protein,Oil and Fatty Acid Compositions in1025 Chinese Soybean Accessions from Diverse Ecoregions
2.1 Introduction
2.1.1 Research objectives
2.2 Materials and methods
2.2.1 Plant materials
2.2.2 Field experiments
2.2.3 Determination of protein and oil content
2.2.4 FA extraction and determination
2.2.5 Geographical distribution mapping
2.2.6 Data analysis
2.3 Results
2.3.1 Variation in seed compositions in1025 Chinese soybean accessions
2.3.2 Accession type affects soybean seed composition
2.3.3 Correlations between soybean seed components
2.3.4 Principal component analysis(PCA)based on ecoregion and accession type
2.3.5 Ecoregion and geographical factors affect soybean seed composition
2.4 Discussion
2.5 Summary
Chapter3 Natural Variation in Seed Protein,Oil and Fatty Acid Compositions of Diverse World Soybean Germplasms Grown in China
3.1 Introduction
3.1.1 Research objectives
3.2 Materials and Methods
3.2.1 Plant materials
3.2.2 Field experiments
3.2.3 Determination of protein and oil contents
3.2.4 FA extraction and determination
3.2.5 Data Analysis
3.3 Results
3.3.1 Variation in seed protein,oil,and FA compositions
3.3.2 Effect of germplasm origins on the variation of seed compositions
3.3.3 Effect of Maturity group on seed compositions
3.3.4 Correlation analysis of soybean seed compositions
3.3.5 Principal components analysis(PCA)
3.3.6 Stability of soybean seed compositions across different environments
3.4 Discussion
3.5 Summary
Chapter4 Candidate Gene Identification of Oil Content Based on BSA-seq Approach in Soybean
4.1 Introduction
4.1.1 Research objectives
4.2 Materials and methods
4.2.1 Plant materials
4.2.2 DNA extraction and extreme oil pools construction
4.2.3 Whole-genome resequencing technology route
4.2.4 Multiple alignment and phylogenetic analyses
4.2.5 Association analysis and geographical distribution
4.3 Results
4.3.1 Variation in seed oil content in a diverse panel of1551 soybean accessions
4.3.2 BSA-seq analysis
4.3.3 SNPs and Indels calling
4.3.4 Functional classification using GO terms and KEGG pathway enrichment analysis
4.3.5 Identification of candidate genes involved in oil metabolic biosynthetic pathway
4.3.6 Identification of protein-truncating variations(PTVs)related to oil biosynthesis
4.3.7 Potential candidate QTL underlying oil content detected using BSA-seq
4.3.8 Natural variation in candidate key genes involved in oil biosynthesis identified by BSA-seq approach
4.3.9 Effect of natural variation in candidate genes on seed oil content
4.3.10 Geographical distribution of oil content owing to DGAT2 and LCAT1 natural variations
4.3.11 Sequence alignment and phylogenetic analysis
4.4 Discussion
4.4.1 BSA-seq and its potential in genetic mapping
4.4.2 Key enzymes involved in FA and TAG biosynthesis successfully identified through the application of BSA-seq approach
4.4.3 The role of PTVs detected in candidate genes involved in oil biosynthesis
4.4.4 Geographical distribution of DGAT2 natural variant in Chinese soybean accessions
4.5 Summary
Chapter5 Conclusion
References
Acknowledgements
Appendixes
Resume
本文编号：3859426
【文章页数】：162 页
【学位级别】：博士
【文章目录】：
摘要
abstract
LIST OF ABBREVIATION
Chapter1 Introduction
1.1 Uses of soybean
1.1.1 Soybean protein
1.1.2 Soybean oil
1.1.3 Soybean FAs
1.2 Soybean origin and domestication
1.3 Effect of environment on soybean seed compositions
1.3.1 Effects of the environment on soybean protein and oil contents
1.3.2 Effects of the environment on soybean seed FA composition
1.4 Effect of maturity on soybean seed compositions
1.5 Metabolic pathway for oil biosynthesis in plants
1.6 Molecular markers
1.7 Next generation sequencing(NGS)and genotyping techniques
1.8 Significance of research work
1.9 Research objective
Chapter2 Profiling of Seed Protein,Oil and Fatty Acid Compositions in1025 Chinese Soybean Accessions from Diverse Ecoregions
2.1 Introduction
2.1.1 Research objectives
2.2 Materials and methods
2.2.1 Plant materials
2.2.2 Field experiments
2.2.3 Determination of protein and oil content
2.2.4 FA extraction and determination
2.2.5 Geographical distribution mapping
2.2.6 Data analysis
2.3 Results
2.3.1 Variation in seed compositions in1025 Chinese soybean accessions
2.3.2 Accession type affects soybean seed composition
2.3.3 Correlations between soybean seed components
2.3.4 Principal component analysis(PCA)based on ecoregion and accession type
2.3.5 Ecoregion and geographical factors affect soybean seed composition
2.4 Discussion
2.5 Summary
Chapter3 Natural Variation in Seed Protein,Oil and Fatty Acid Compositions of Diverse World Soybean Germplasms Grown in China
3.1 Introduction
3.1.1 Research objectives
3.2 Materials and Methods
3.2.1 Plant materials
3.2.2 Field experiments
3.2.3 Determination of protein and oil contents
3.2.4 FA extraction and determination
3.2.5 Data Analysis
3.3 Results
3.3.1 Variation in seed protein,oil,and FA compositions
3.3.2 Effect of germplasm origins on the variation of seed compositions
3.3.3 Effect of Maturity group on seed compositions
3.3.4 Correlation analysis of soybean seed compositions
3.3.5 Principal components analysis(PCA)
3.3.6 Stability of soybean seed compositions across different environments
3.4 Discussion
3.5 Summary
Chapter4 Candidate Gene Identification of Oil Content Based on BSA-seq Approach in Soybean
4.1 Introduction
4.1.1 Research objectives
4.2 Materials and methods
4.2.1 Plant materials
4.2.2 DNA extraction and extreme oil pools construction
4.2.3 Whole-genome resequencing technology route
4.2.4 Multiple alignment and phylogenetic analyses
4.2.5 Association analysis and geographical distribution
4.3 Results
4.3.1 Variation in seed oil content in a diverse panel of1551 soybean accessions
4.3.2 BSA-seq analysis
4.3.3 SNPs and Indels calling
4.3.4 Functional classification using GO terms and KEGG pathway enrichment analysis
4.3.5 Identification of candidate genes involved in oil metabolic biosynthetic pathway
4.3.6 Identification of protein-truncating variations(PTVs)related to oil biosynthesis
4.3.7 Potential candidate QTL underlying oil content detected using BSA-seq
4.3.8 Natural variation in candidate key genes involved in oil biosynthesis identified by BSA-seq approach
4.3.9 Effect of natural variation in candidate genes on seed oil content
4.3.10 Geographical distribution of oil content owing to DGAT2 and LCAT1 natural variations
4.3.11 Sequence alignment and phylogenetic analysis
4.4 Discussion
4.4.1 BSA-seq and its potential in genetic mapping
4.4.2 Key enzymes involved in FA and TAG biosynthesis successfully identified through the application of BSA-seq approach
4.4.3 The role of PTVs detected in candidate genes involved in oil biosynthesis
4.4.4 Geographical distribution of DGAT2 natural variant in Chinese soybean accessions
4.5 Summary
Chapter5 Conclusion
References
Acknowledgements
Appendixes
Resume
本文编号：3859426
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