多菌种协同分解中低品位磷矿的作用机理研究

发布时间：2018-03-03 21:33

本文选题：中低品位磷矿　切入点：混合菌　出处：《武汉工程大学》2015年硕士论文　论文类型：学位论文

【摘要】：本文以多菌种协同分解中低品位磷矿为主线,从以下三个方面展开了研究,以期为解决我国丰富的中低品位磷矿资源利用难题提供理论依据。1.多菌种协同分解中低品位磷矿的探索分别以两种不同来源(小麦根际土壤、城市废水厂的活性污泥)的混合菌及两种云南中低品位磷矿为研究对象,研究了矿石粒度及矿浆浓度对微生物分解中低品位磷矿的影响,并分析了作用过程中,菌种生物量、pH值及解磷量之间的相关性。结果表明,在初始pH 7.0,反应温度25℃,转速为200 r/min的条件下,当矿石粒度分别为150μm,矿浆浓度为1%时,混合菌对中低品位磷矿的作用效果较好;作用过程中,培养液中的菌种生物量、pH值与解磷量间存在相关性,即菌种生物量与解磷量间表现为正相关,pH值与解磷量间表现为负相关。2.多菌种在浸取柱中协同分解中低品位磷矿以源于城市废水厂的活性污泥未经分离、筛选过程得到的混合菌及云南某中低品位磷矿为研究对象,通过柱浸装置研究了微生物对中低品位磷矿的分解作用。结果表明,以柱浸装置研究微生物与中低品位磷矿的作用是可行的,作用过程中,出水样中的菌种量逐渐增加,pH值维持在酸性范围内,解磷量逐渐增加。作用60-67d,进出水化学需氧量(COD)测试结果表明,柱中混合菌的生长需要消耗大量的有机物,作用过程中,应当提供足够的碳源以满足菌种生长的需要。生物膜中pH及溶解氧的测定结果表明,生物膜中的pH(4-5左右)与出水样中的pH基本保持一致。当距离生物膜表面1100μm时,氧气几乎耗尽,生物膜中耗氧层与缺氧层并存。作用30 d及90 d,矿物表面扫描电子显微镜(SEM)测试结果表明,随着作用时间的增加,生物膜逐渐覆盖矿物表面,并破坏了矿物表面结构。群落分析结果表明,作用60 d后,出水样与生物膜中的群菌差异较大。出水样中的主要菌群是Alphaproteobacteria(63.19%),相较之下,生物膜中的菌群多样性较大,含量分布较分散。3.多菌种在生物反应器中协同分解中低品位磷矿分别以两种不同来源(小麦根际土壤、城市废水厂的活性污泥)的混合菌及两种云南中低品位磷矿为研究对象,通过生物反应器装置,研究了混合菌对中低品位磷矿的作用,并比较了不同中低品位磷矿与不同混合菌之间的作用效果。结果表明,两种不同来源的混合菌均能有效分解这两种中低品位磷矿。当同一来源的混合菌对不同的矿物作用时,混合菌对矿1的分解效果更为显著。不同来源的混合菌对同一矿物作用时,源于活性污泥的混合菌分解中低品位磷矿的效果更佳。矿物表面SEM测试结果表明,菌种对矿物的表面结构有一定的破坏作用。群落分析结果表明,混合菌与矿物作用30 d后,培养液中主要的菌种类型相似。但是不同的培养环境,其含量差异较大。土壤菌与矿物作用时,Alphaproteobacteria和TM7_class_incertae_sedis在土壤菌与矿2作用时含量较高;活性污泥菌与矿2作用时,TM7_class_incertae_sedis(34.52%),Gammaproteobacteria(19.84%)含量较活性污泥菌与矿1作用时高。
[Abstract]:Based on the multi strain co low grade phosphate rock decomposition as the main line, study is carried out from the following three aspects, in order to solve the rich medium and low grade phosphate rock resources utilization in China and provide a theoretical basis for the problem of.1. multi strain co exploration low grade phosphate rock decomposition respectively with two different sources of activated sludge (city wastewater treatment plant in wheat rhizosphere soil) mixed bacteria and two kinds of Yunnan low grade phosphate rock as research object, studies the ore particle size and pulp concentration on microbial effects of low grade phosphate rock decomposition, and analyzes the role in the process of fungal biomass, the correlation between pH value and P content. The results showed that in the initial pH 7, reaction temperature 25 degrees, speed is 200 r/min, when the ore particle size of 150 m respectively, the pulp concentration is 1%, the better the effect of mixed bacteria of low grade phosphate rock; the process, in the culture medium of bacteria Biomass and P content, the correlation between pH value, namely the fungal biomass and P content showed positive correlation, pH value and P content showed a negative correlation between the.2. strains in the extraction column in collaborative low grade phosphate rock decomposition to activated sludge in wastewater plant source city without separation the screening process, mixed bacteria and a Yunnan medium and low grade phosphate rock as the research object, studied the microbial decomposition effect of low grade phosphate rock by column leaching device. The results show that the column leaching device of microorganisms and low grade phosphate rock effect is feasible, the role in the process of the bacteria in water gradually increased and the pH value is maintained in the acidic range, P content increased gradually. The role of 60-67d, the import of chemical oxygen demand (COD) test results showed that the column growth of mixed bacteria need to consume a large amount of organic matter, the process, should provide sufficient carbon source In order to meet the needs of the growth of strain. The testing results of pH and dissolved oxygen in the biofilm showed that biofilm in pH (about 4-5) and water in pH remained the same. When the distance between the biofilm surface 1100 m, oxygen was almost exhausted, the membrane layer and the oxygen consumption for anoxic layer coexist. With 30 d and 90 d, scanning electron microscopy (SEM) mineral surface test results show that with the time increasing, the biofilm gradually covered the mineral surface, and destroyed the structure of mineral surface. Cluster analysis results show that after 60 d, a group of bacteria in the biofilm samples and the differences of main bacteria greatly. In the samples of group Alphaproteobacteria (63.19%), in contrast, in the biofilm bacteria diversity in larger, more dispersed distribution low grade phosphate rock decomposition by two different sources of collaborative.3. strains in the bioreactor (wheat rhizosphere soil, city wastewater treatment plant The activated sludge) mixed bacteria and two kinds of Yunnan low grade phosphate rock as the research object, through the biological reactor, study the effect of mixed bacteria of low grade phosphate rock, and compare the effect between different medium and low grade phosphate rock with different mixed bacteria. The results showed that two different sources of mixed bacteria are effective the decomposition of these two kinds of medium and low grade phosphate rock. When the mixed bacteria of the same source of different minerals, mixed bacteria on the decomposition effect of mine 1 was more significant. The role of mixed bacteria from different sources on the same mineral, the better effect of activated sludge mixed bacteria from low grade phosphate rock decomposition. The mineral surface SEM the test results show that the surface structure of the mineral species were affected communities. The analysis results show that the mixed bacteria and minerals for 30 d, the main types of liquid culture were similar. But the different culture ring Exit, its content is different. Soil bacteria and mineral effect, Alphaproteobacteria and TM7_class_incertae_sedis in soil bacteria and 2 when high ore content; active sludge and mineral 2, TM7_class_incertae_sedis (34.52%), Gammaproteobacteria (19.84%) compared with the content of active sludge and coal mine 1 high effect.

【学位授予单位】：武汉工程大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TD925.5;TD97

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