淮北任楼煤矿灰岩异常水质的菌群，气体成因分析

发布时间：2018-06-20 11:00

本文选题：太灰水 + 数据分析　；参考：《合肥工业大学》2017年硕士论文

【摘要】：针对任楼煤矿水质异常情况,结合周边煤矿进行现场调研,采集水样、测定分析其常规离子,并开展了井下钻孔水样细菌高通量基因测序,运用piper三线图、聚类、因子分析、灰色关联度分析等多方面数据分析,发现在封闭性较高环境中,太灰水呈现出向煤系水过渡的特点,水中Ca~(2+)与Mg~(2+)减少,矿化度下降,pH增大。基因测序结果显示深部太灰水含大量微好氧及厌氧细菌,检出的Desulfobacterales目、产甲烷古菌等厌氧产气细菌证明微生物水文地球化学作用是影响煤矿地下水水质的原因之一。由于断层上下盘地层错动引起局部水循环条件差、较高封闭性和低氧化还原电位,任楼中六运输大巷太原组灰岩水缺乏与其他灰岩水的水力联系,导致阳离子交替吸附、硫酸盐还原作用发育,从而使局部地区地下水呈现高HCO_3~-低SO_4~(2-),高K~+、Na~+低Ca~(2+)、Mg~(2+),地下水硬度也随之减小的特点。为验证地下水中的硫酸盐还原作用和产甲烷作用,对任楼中六运输大巷钻孔出水水样的细菌采用高通量测序,检测出大量的产甲烷菌和硫酸盐还原菌。通过基因序列对比发现,两淮矿区深层地下水中脱硫酸细菌和产甲烷古菌含量丰富,这意味着在煤田地下水中持续发生着微生物型硫化氢与甲烷气体生成作用。对矿井煤层气进行检测发现,烃类气体以CH_4为主,重烃气体检测到乙烷和丙烷,非烃类气体中CO_2浓度为小于5%,属于典型的生物成因气。
[Abstract]:According to the abnormal water quality of Ren Lou coal mine, combined with the surrounding coal mine field investigation, collecting water sample, measuring and analyzing its conventional ions, and carrying out the high throughput gene sequencing of borehole water sample bacteria, using Piper three line map, clustering, factor analysis, grey correlation analysis and other aspects of data analysis, found that in a high closed environment, too high environment, too high. Ca~ (2+) and Mg~ (2+) in water decreased, mineralization decreased and pH increased. The results of gene sequencing showed that the deep too ash water contained a large number of microaerobic and anaerobic bacteria, the detected Desulfobacterales mesh, methane producing bacteria and other anaerobic bacteria producing bacteria, which proved that the microbial hydrogeochemical effect was influenced by the underground coal mine underground One of the reasons for water quality is that the faults on the upper and lower layers of the fault cause poor local water circulation conditions, high sealing and low oxidation-reduction potential, and the hydraulic connection between the limestone water of the Taiyuan group in the six transportation big Lane in the Ren Lou and the other limestone water, resulting in the alternate cation adsorption and the development of the sulphate reduction, thus making the local groundwater The characteristics of high HCO_3~- low SO_4~ (2-), high K~+, Na~+ low Ca~ (2+), Mg~ (2+), and groundwater hardness also decrease. In order to verify the sulfate reduction and methane production in groundwater, a high throughput sequencing was used to detect the bacteria of the water samples in the six lanes of the big Lane in the building, and a large number of methanogenic bacteria and sulfate reducing bacteria were detected. It is found that the content of desulfuric acid bacteria and methanogenic bacteria in deep groundwater in the two Huaihe mining area is rich, which means that the microbial type hydrogen sulfide and methane gas are occurring in the underground water of the coal field. The detection of coal mine gas in coal mine shows that the hydrocarbon gases are mainly CH_4, and the heavy hydrocarbon gases detect ethane and propane. The concentration of CO_2 in non hydrocarbon gas is less than 5%, which is a typical biogenic gas.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：P641.461

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