山东地区海水养殖区常见抗生素耐药菌及耐药基因分布特征

发布时间：2018-01-08 04:32

本文关键词：山东地区海水养殖区常见抗生素耐药菌及耐药基因分布特征　出处：《海洋环境科学》2016年01期 　论文类型：期刊论文

【摘要】：对山东地区海水养殖海域常见抗生素耐药菌及耐药基因分布进行调查,结果表明所调查5个海水养殖区水样中四环素类、磺胺类、β-内酰胺类耐药菌比例显著(p0.05)高于氯霉素类和喹诺酮类耐药菌;此外用RT-PCR方法对共计15种耐药基因在水样中的丰度测定表明磺胺类(sul、dfra/16S rRNA=10~(-6)~10~(-2))、喹诺酮类(qnr/16S rRNA=10~(-6)~10~(-2))以及四环素类耐药基因(tet/16S rRNA=10~(-7)~10~(-2))在各水样中丰度差异不显著,而氯霉素耐药基因(cata、cmle/16S rRNA=10~(-8)~10~(-2))在不同水样中丰度差异显著,且cata1和cmle1丰度与可培养氯霉素耐药菌比例存在相关线性关系。实验数据说明山东海水养殖区水样中存在一定的抗生素耐药菌和耐药基因污染。
[Abstract]:The distribution of common antibiotic resistant bacteria and drug resistance genes in marine culture area of Shandong Province was investigated. The results showed that tetracycline and sulfanilamide in water samples from 5 marine culture areas were investigated. The proportion of 尾 -lactam resistant bacteria was significantly higher than that of chloramphenicol and quinolone resistant strains (P 0.05). In addition, the abundance of a total of 15 resistant genes in water samples was determined by RT-PCR method. Dfra/16S rRNAs 10 ~ (6) ~ 10 ~ (~ (2)). Quinolones Qnr / 16s rRNAs 10nnrRNAs 10nrRNAs 10nrRNAs, and tetracycline resistance gene tetr / 16s rRNA10rRNAs 10nrRNAs / 7rRNAs (QNR / 16s rRNAs) and tetracycline resistance gene (tetr / 16s rRNAs). There was no significant difference in abundance between different water samples. However, the chloramphenicol resistance gene, cata1 / 16s rRNA1, was significantly higher in different water samples than that in the control group (P < 0.05), but the abundance of Chloramphenicol resistance gene was significantly different in different water samples. There was a linear correlation between the abundance of cata1 and cmle1 and the proportion of culturable chloramphenicol resistant bacteria.
【作者单位】：中国海洋大学生物多样性与进化研究所;国家海洋局第一海洋研究所;
【基金】：国家海洋公益性行业科研专项资助项目(201105007-1) 国家科技支撑计划项目(2012BAD17B01)
【分类号】：X714;X55
【正文快照】： 266061)抗生素药物在养殖环境中因预防或治疗水产动物细菌性疾病而大量使用已造成环境中抗生素耐药菌的泛滥。这些抗生素耐药菌通常携带有一种或多种抗生素耐药基因,由于耐药基因的横向传递作用[1],耐药基因可以从非致病性细菌转移到病原菌中或随着食物链的传递转移到人或其他

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