应用PLFA技术分析氮沉降对三江平原小叶章湿地土壤微生物群落结构的影响

发布时间：2018-01-12 09:13

本文关键词：应用PLFA技术分析氮沉降对三江平原小叶章湿地土壤微生物群落结构的影响　出处：《中南林业科技大学学报》2016年12期 　论文类型：期刊论文

【摘要】：为了研究氮沉降对三江湿地土壤微生物群落结构的影响,利用磷脂脂肪酸技术(PLFA)对三江平原小叶章湿地土壤微生物多样性进行了分析。2010年5月,在黑龙江省科学院自然与生态研究所三江平原湿地生态定位研究站内的小叶章湿地中建立模拟氮沉降试验平台,设置3个氮沉降浓度梯度:N1(0 g N·m-2a-1)、N2(4 g N·m-2a-1)、N3(8 g N·m-2a-1),于2014年6月用土钻进行土壤样品采集。结果表明:本研究共检测到PLFAs 75种,其中特征脂肪酸29种。不同氮沉降处理下,真菌群落的生物量较高,土壤微生物生物量的总量介于30~33nmol·g-1。氮沉降增加对真菌群落数量的影响比较显著(P0.05),但是对细菌和放线菌影响并不显著。低氮时土壤微生物的数量最多,对照最低;高氮时土壤微生物群落的多样性值和丰度值最高。根据典型性相关分析,得出铵态氮和硝态氮对土壤中真菌含量影响较为显著。通过本研究可以得出,施氮增加了土壤微生物总量。铵态氮和硝态氮是影响土壤微生物数量的主要因子,低氮增加了土壤微生物多样性,高氮则产生抑制作用。
[Abstract]:In order to study the effect of nitrogen deposition on soil microbial community structure in Sanjiang wetland. The microbial diversity in the soil of the small leaf chapter wetland in Sanjiang Plain was analyzed by using phospholipid fatty acid technique (PLFAA). May 2010. An experimental platform for simulating nitrogen deposition was established in the wetland ecological location station of Sanjiang Plain of Heilongjiang Academy of Sciences. Three nitrogen deposition gradient of N _ (1) 0 g N 路m ~ (-2) a ~ (-1) N ~ (2 +) ~ (4 g N 路m ~ (-2) a ~ (-1) N _ 3N _ (3) N 路m ~ (-2) a ~ (-1)). In June 2014, soil samples were collected with soil drill. The results showed that 75 species of PLFAs were detected in this study, including 29 species of characteristic fatty acids. The biomass of fungi community was higher, and the total biomass of soil microorganism was between 30 ~ 33nmol 路g ~ (-1). The effect of nitrogen deposition on the quantity of fungi community was significant (P0.05). But the effect on bacteria and actinomycetes was not significant. The diversity and abundance of soil microbial communities were the highest when nitrogen was high. According to the typical correlation analysis, the effects of ammonium nitrogen and nitrate nitrogen on the soil fungal content were significant. Ammonium nitrogen and nitrate nitrogen were the main factors affecting soil microbial quantity, low nitrogen increased soil microbial diversity, and high nitrogen had inhibitory effect.
【作者单位】：东北林业大学林学院;黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室;
【基金】：国家自然科学基金项目(31470019,31400429,31500410) 十三五国家重点研发计划(2016YFC0500405) 黑龙江省科学院项目(STJB16-01,STJB16-04,2015YZ01,2014ST05) 黑龙江省博士后基金(LBH-Z14184) 黑龙江省科研机构创新能力提升专项计划(GY2014KC0039) 黑龙江省科学院青年创新基金资助
【分类号】：S154.3
【正文快照】： (1.College of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China;2.National and Provincial Joint EngineeringLaboratory of Wetlands and Ecological Conservation Institute of NatureEcology,Heilongjiang Academy of Sciences,Harbin 150040

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