全基因组预测枯草芽孢杆菌XF-1的分泌蛋白

发布时间：2018-01-12 22:05

本文关键词：全基因组预测枯草芽孢杆菌XF-1的分泌蛋白　出处：《福建农业学报》2016年04期 　论文类型：期刊论文

【摘要】：枯草芽孢杆菌XF-1对诸多植物病原菌具有抑制作用,是农业生产上重要的生防菌之一。利用SignalP、ProtComp、TMHMM、Phobius、LipoP、TatP等预测程序对枯草芽孢杆菌中3 853条蛋白质序列进行分泌蛋白的预测研究,并对所获得的分泌蛋白开展氨基酸分布、信号肽长度、切割位点等性质进行分析。结果表明,该菌含有分泌蛋白为104个,其氨基酸长度、信号肽长度与植物病原菌不同;信号肽切割位点属于A-X-A类型,与其他已经报道的植物病原真菌、细菌以及卵菌中分泌蛋白信号肽切割位点一致。通过上述生物信息学分析方法有效地实现了枯草芽孢杆菌分泌蛋白的预测,分泌蛋白的信号肽切割位点具有物种保守型特点。
[Abstract]:Bacillus subtilis XF-1 (Bacillus subtilis) has inhibitory effect on many plant pathogens and is one of the most important biocontrol bacteria in agricultural production. Three hundred and fifty-three protein sequences of Bacillus subtilis were predicted by Phobius-LipoP TatP program. The amino acid distribution, signal peptide length and cleavage site of the secreted protein were analyzed. The results showed that the secreted protein was 104, and the amino acid length of the secreted protein was 104. The length of signal peptide was different from that of plant pathogen. The signal peptide cleavage site belongs to A-X-A type and is associated with other plant pathogenic fungi that have been reported. The cleavage sites of secretory protein signal peptides in bacteria and oocytes were the same. The prediction of secretory proteins of Bacillus subtilis was effectively realized by the bioinformatics analysis method mentioned above. The signal peptide cleavage sites of secretory proteins are species conserved.
【作者单位】：西南林业大学林学院/云南省森林灾害预警与控制重点实验室;
【基金】：国家自然科学基金项目(31560211) 云南省森林灾害预警与控制重点实验室开放基金项目(ZK150004) 云南省优势特色重点学科生物学一级学科建设项目(50097505) 云南省高校林下生物资源保护及利用科技创新团队建设项目(2014015)
【分类号】：S476.1
【正文快照】： 枯草芽孢杆菌Bacillus subtilis XF-1是云南农业大学于2006年从土壤中筛选获得的,其对十字花科根肿病具有较强的抑制作用[1],同时,也对马铃薯晚疫病菌、玉米小斑病菌、稻瘟病菌等21种植物病原菌具有较好的抑制作用[2]。目前,前人已对该菌在大白菜根际的定殖能力[3-4]、功能蛋

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