Cd胁迫下外生菌根菌对樟子松生理指标和根际土壤酶的影响

发布时间：2018-01-24 16:20

本文关键词： 外生菌根菌樟子松生理指标土壤酶　出处：《生态学杂志》2017年11期 　论文类型：期刊论文

【摘要】：本研究采用盆栽试验法,研究了不同浓度Cd胁迫下,接种外生菌根菌——褐环乳牛肝菌(Suillus luteus)对樟子松(Pinus sylvestris var.mongolica)苗期植株的生长及叶绿素含量、抗氧化酶(CAT、POD和SOD)活性、细胞渗透调节物质(可溶性糖、MDA和游离脯氨酸)含量和根际土壤酶(过氧化氢酶、脲酶和碱性磷酸酶)活性的变化。结果表明:1年生樟子松苗木在Cd胁迫1个月后,接菌处理组无论在苗木生长、抗氧化酶活性方面均高于对照组,且大部分指标达到显著差异水平(P0.05)。各接菌处理组菌根侵染率达48.0%以上。抗氧化酶活性方面,CAT和POD活性呈现出随着胁迫程度的加深,先下降后上升的变化趋势,SOD活性则呈现出地上与地下部分"上下对称"的变化趋势。同时,通过对各处理组苗木细胞渗透调节物质的定量测定,接菌处理组的细胞受破坏程度显著低于不接菌处理组。对苗木根际土壤酶分析结果表明,Cd胁迫下,施加外生菌根菌可以有效提高樟子松根际土壤酶的活性(尤其是脲酶),进而起到增加樟子松根际土壤生物活性作用。综上,接种外生菌根真菌对樟子松缓解重金属Cd胁迫具有明显的促进作用。Cd胁迫下,外生菌根可以有效正向调节樟子松的生长、抗氧化胁迫能力并提高苗木细胞渗透调节作用,进而提高苗木对重金属的耐受性和提高苗木根际土壤生物活性。本研究可以为重金属污染土地的有效治理和利用木本植物修复改良重金属污染土壤提供参考。
[Abstract]:In this study, pot experiment was used to study the effects of CD stress on different concentrations of CD. Inoculation of ectomycorrhizal fungi, Suillus luteus, against Pinus sylvestris var. mongolica. Seedling growth and chlorophyll content. The activities of antioxidant enzymes such as POD and SOD, the content of cellular osmotic regulators (soluble sugar malondialdehyde (MDA) and free proline) and the rhizosphere soil enzymes (catalase) were observed. The changes of urease and alkaline phosphatase activities. The results showed that the activities of antioxidant enzymes in seedlings of 1-year-old Pinus sylvestris var. mongolica seedlings were higher than those of control group after 1 month of CD stress. And most of the indexes reached significant difference level (P0.05). The mycorrhizal infection rate of each inoculation group was more than 48.0%, and the antioxidant enzyme activity was more than 48.0%. The activity of CAT and POD showed a tendency of decreasing first and then rising with the deepening of stress, while the activity of CAT showed a tendency of "up and down symmetry" between above and below. Through the quantitative determination of osmotic regulatory substances in seedling cells of each treatment group, the degree of cell damage in inoculated group was significantly lower than that in non-inoculated group. The results of enzyme analysis in rhizosphere soil of seedlings showed that under CD stress. The application of ectomycorrhizal fungi can effectively increase the soil enzyme activity (especially urease) of Pinus sylvestris var. mongolica rhizosphere, and then increase the biological activity of rhizosphere soil of Pinus sylvestris var. mongolica. Inoculation of ectomycorrhizal fungi could significantly promote the alleviation of CD stress of Pinus sylvestris var. mongolica. Under CD stress, ectomycorrhizal fungi could positively regulate the growth of Pinus sylvestris var. mongolica. The ability of antioxidant stress and the osmotic regulation of seedling cells were improved. This study can provide a reference for the effective treatment of heavy metal contaminated land and the remediation and improvement of heavy metal contaminated soil by woody plants.
【作者单位】：沈阳农业大学林学院;黑龙江省林科院森林保护研究所;
【基金】：沈阳农业大学引进人才启动项目(2015年) 黑龙江林科院人才培养基金项目(2015R01) 国家自然科学基金项目(31670649,31200484,31700564) 黑龙江省级基本科研业务费项目(黑龙江省财政厅项目)资助
【分类号】：S714.3;S791.253
【正文快照】： 镉(Cd)污染是众多的环境问题当中尤为重要的一类(刘广深等,2004;徐明岗等,2008)。土壤中的Cd主要来自化学工业排放的废水,城市垃圾的燃烧(He et al.,2013),含重金属农药的使用等(崔玉静等,2003;李静等,2008)。Cd对植物具有毒性,影响植物的生理生化过程(Sobkowiak et al.,2004;

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