一株烟草秸秆降解菌的分离、鉴定及酶学性质研究

发布时间：2018-01-30 10:51

本文关键词： 枯草芽孢杆菌纤维素酶产酶特性　出处：《土壤》2016年05期 　论文类型：期刊论文

【摘要】：从皖南地区烟稻轮作田土壤中经CMC-Na初筛获得5株纤维素降解菌,经DNS法测定纤维素酶活性复筛得到一株降解活性较高的降解菌YC-2。根据该菌16S r DNA序列比对结果,结合形态和生理生化特征,确定YC-2为枯草芽孢杆菌(Bacillus subtilis)。对YC-2酶学性质进行相关研究,并分析YC-2对烟碱的耐受情况及对烟草秸秆的降解情况,结果表明:在7天内,YC-2对烟草秸秆降解率为10.14%;酶学特性表现为最适反应温度为60℃且在15~60℃之间具有稳定性;最适反应pH为7.0,在pH 4.0~7.5范围内稳定性较好;YC-2在浓度为1~2 g/L烟碱中能快速生长,而在高浓度的烟碱中生长受到抑制。因此,菌株YC-2产纤维素酶活性较高、相对耐热耐碱且对烟杆有一定分解作用,通过进一步诱变选育和发酵条件优化有较好的田间应用潜力。
[Abstract]:From the southern Anhui Tobacco rice rotation in soil by CMC-Na screening 5 cellulose degrading bacteria by DNS method for the determination of cellulase activity screening of degrading bacteria YC-2. had a higher activity of degrading bacteria 16S R DNA according to the sequence alignment results, combined with the physiological and biochemical characteristics, YC-2 was identified as Bacillus subtilis (Bacillus subtilis). The research of YC-2 enzymatic properties, and analyze the tolerance of YC-2 on nicotine and degradation of tobacco straw, results showed that: in 7 days, YC-2 on tobacco straw degradation rate was 10.14%; enzymatic properties showed the optimum reaction temperature is 60 DEG and 15~60 DEG with the optimum stability; the reaction of pH was 7, better stability in the range of 4.0~7.5 pH; YC-2 1~2 g/L at the concentration of nicotine can rapid growth, while higher concentration of nicotine in growth was inhibited. Therefore, strain YC-2 producing high cellulase activity. It has a certain decomposition effect on heat resistance and alkali resistance, and has good potential for field application by further mutation breeding and optimization of fermentation conditions.

【作者单位】：南京农业大学生命科学学院/农业部农业环境微生物工程重点开放实验室;安徽省农业科学院烟草研究所;
【分类号】：S154.39
【正文快照】： 自然界中,纤维素可占植物体总质量40%左右[1]。然而,由于植物秸秆结构的特殊性(纤维素构成细胞壁,其间缠绕具有空间结构的半纤维素及木质素)而导致微生物很难降解秸秆纤维素[2]。作为农业生产大国,中国每年秸秆产量超过6亿t[3],除秸秆还田[4]外,秸秆气化、热解发电、秸秆青贮

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