玉米芯、竹炭及油枯吸附-海藻酸钠包埋施氏假单胞菌PFS-4对二氯喹啉酸的降解

发布时间：2018-02-03 16:29

  本文关键词： 二氯喹啉酸 玉米芯、竹炭及油枯复合载体 固定化 生物修复　出处：《应用生态学报》2017年02期 　论文类型：期刊论文

【摘要】：采用玉米芯、竹炭及油枯吸附-海藻酸钠包埋对分离到的施氏假单胞菌PFS-4进行复合固定.采用正交试验对固定化条件进行优化,研究了固定化菌剂及游离菌体对二氯喹啉酸的降解效果.结果表明:固定化菌剂制备的最佳条件为:海藻酸钠质量分数为4%、吸附载体比例(玉米芯∶竹炭∶油枯)为1∶2∶1、CaCl_2质量分数为3%、交联时间4 h.固定化菌剂在温度为30℃、初始pH=7的条件下,经6 d培养后,对浓度为800 mg·L~(-1)的二氯喹啉酸降解率为91.4%,而游离菌体的降解率为72.8%.将游离菌体和固定化菌剂用于实际污水及土壤处理时,固定化菌剂对水中及土壤中二氯喹啉酸去除率仍能分别达到84.2%和74.3%.研究结果表明,载体及其联结方式对土壤中二氯喹啉酸去除产生显著影响,翻动频率与土壤中二氯喹啉酸的去除率呈显著正相关.因此,玉米芯、竹炭及油枯吸附-海藻酸钠复合固定施氏假单胞菌PFS-4对不良环境具有较好的缓冲性能,对二氯喹啉酸污染水体及土壤原位生态修复具有潜力.
[Abstract]:Corn cob, bamboo charcoal and oil withered adsorption-sodium alginate were used to immobilize the isolated Pseudomonas sp. PFS-4. Orthogonal experiment was used to optimize the immobilization conditions. The degradation of dichloroquinolinic acid by immobilized bacteria and free bacteria was studied. The results showed that the optimum conditions for the preparation of immobilized bacteria were as follows: sodium alginate 4%. The adsorption carrier ratio (corn cob: bamboo charcoal: oil withered) was 1: 2: 1: 1 CaCl2 mass fraction was 3, crosslinking time was 4 h. The immobilized bacteria agent was immobilized at 30 鈩，

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