过氧化氢和一氧化氮在小球藻抗阿特拉津胁迫中的作用

发布时间：2018-02-11 07:47

本文关键词： 阿特拉津小球藻 CAT 一氧化氮过氧化氢 PTIO　出处：《生态毒理学报》2016年04期 　论文类型：期刊论文

【摘要】：过氧化氢(H_2O_2)和一氧化氮(NO)作为信号分子,可调节植物生长、发育以及应对外源性胁迫。利用过氧化氢酶(CAT)以及NO清除剂(PTIO),研究了除草剂阿特拉津(atrazine,100μg·L~(-1))影响小球藻生长的机理,并分析内源性H_2O_2和NO在小球藻抗除草剂胁迫中的作用。研究结果表明,阿特拉津在诱发小球藻细胞死亡的过程中,不同程度促发了H_2O_2和NO生成;外源CAT可通过清除H_2O_2和诱导NO来缓解阿特拉津对小球藻的生长抑制;PTIO与阿特拉津的联合实验进一步证实,小球藻体内的NO诱导与H_2O_2的爆发无关,它们之间的合成没有相关性。因此,除草剂阿特拉津主要通过诱导小球藻体内的H_2O_2爆发来破坏藻细胞,抑制其生长,与NO的信号传递无关。
[Abstract]:The effects of the herbicide atrazine (100 渭 g 路L ~ (-1)) on the growth of Chlorella microphylla were studied by means of catalase CAT) and no scavenger (PTIOION), which acted as signal molecules to regulate plant growth, development and response to exogenous stress, and to investigate the effects of atrazine (100 渭 g 路L ~ (-1)) on the growth of Chlorella microphylla by using catalase (CAT) and no scavenger (PIO). The effects of endogenous H _ 2O _ 2 and no on the herbicide resistance of Chlorella vulgaris were analyzed. The results showed that atrazine promoted the production of H _ 2O _ 2 and no in different degrees during the process of inducing the cell death of Chlorella vulgaris. Exogenous CAT could relieve the growth inhibition of Chlorella vulgaris by removing H2O2 and inducing no. The results further confirmed that no induction in Chlorella sp. Had nothing to do with the outbreak of H2O2 in Chlorella sp. Therefore, the herbicide atrazine mainly destroys algal cells and inhibits their growth by inducing Hs _ 2O _ 2 outburst in Chlorella vulgaris, and has nothing to do with signal transduction of no.
【作者单位】：浙江工业大学海洋学院;浙江工业大学生物工程学院;浙江工业大学环境学院;
【基金】：国家自然科学基金(21577128) 浙江省大学生科技创新活动计划(新苗人才计划)
【分类号】：X592;X173

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