基于宏基因组学16S rDNA测序对烟草根际土壤细菌群落组成分析

发布时间：2018-02-19 18:43

本文关键词： 烟草根际土壤细菌宏基因组学冗余度分析　出处：《中国农业科技导报》2017年02期 　论文类型：期刊论文

【摘要】：为了研究烟草根际土壤细菌多样性,了解其细菌群落的组成和结构,从而为健康植烟土壤生态系统的构建和保持提供理论依据和指导。以2014年四川凉山州健康烟株现蕾期根际土壤为研究样本,利用Illumina平台Miseq高通量测序技术对烟草根际土壤细菌16S rDNA进行高通量测序分析。结果表明:烟草根际土壤微生物多样性水平较高,不同样品间主要菌落分布结构相对稳定。在门分类水平上,共检测分类25个菌门,其中变形菌门占有明显优势(39.04%~53.71%),其次为拟杆菌门、酸杆菌门、疣微菌门;在属分类水平上,共检测分类576类菌属,鞘脂单胞菌属、Flavisolibacter、Ohtaekwangia为烟草根际土壤细菌明显优势菌属。大部分主要菌群之间存在明显正相关关系,对烟草根际土壤细菌影响较大的环境因子为土壤速效氮、含水量和pH。运用宏基因组学16S rDNA测序技术获取的烟草根际土壤细菌信息较为丰富,为健康植烟土壤机制研究提供新的理论参考。
[Abstract]:In order to study bacterial diversity in tobacco rhizosphere soil and understand the composition and structure of bacterial community, So as to provide theoretical basis and guidance for the construction and maintenance of healthy tobacco planting soil ecosystem. In 2014, the rhizosphere soil of healthy tobacco plants in Liangshan Prefecture, Sichuan Province, was taken as the research sample. The high throughput sequencing analysis of tobacco rhizosphere bacteria 16s rDNA was carried out by using Miseq high-throughput sequencing technique based on Illumina platform. The results showed that the microbial diversity of tobacco rhizosphere soil was higher than that of tobacco rhizosphere soil. The distribution structure of the main colonies was relatively stable among different samples. At the level of phyla, 25 phylum were detected, among which Proteus had a significant advantage in 53.71G, followed by Bacteroides, acidomycetes, Microphylum verruca, and at the taxonomic level. A total of 576 species of bacteria were classified, and the genus Flavisolibacter Ohtaekwangia was the dominant genus of bacteria in tobacco rhizosphere soil. There was a significant positive correlation between most of the major bacterial groups, and the most important environmental factor affecting bacteria in rhizosphere of tobacco was soil available nitrogen. The microbial information of rhizosphere soil obtained by macrogenomics 16s rDNA sequencing technique is abundant, which provides a new theoretical reference for the study of soil mechanism of healthy tobacco planting.
【作者单位】：河南农业大学烟草学院;江西省烟草公司抚州市公司;
【基金】：河南省烟草公司资助项目(HYKJ201301)资助
【分类号】：S154.3;S572

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