秋茄内生细菌多样性及抑制甘蔗黑穗霉菌活性研究

发布时间：2018-02-26 08:54

本文关键词： 秋茄内生细菌物种多样性甘蔗黑穗霉菌抑菌活性　出处：《中国抗生素杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的系统地对广西北海金海湾红树植物秋茄(Kandelia candel)内生细菌的分布特征与物种多样性进行分析,开展细菌代谢粗提物对甘蔗黑穗霉菌的抑制活性研究,为开发新型生物类农药及生物膜制剂奠定基础。方法采用7种分离培养基,从秋茄各部位组织中分离可培养细菌;通过16S rRNA基因序列的系统发育学分析其物种多样性。采用牛津杯法研究内生细菌对甘蔗黑穗霉菌的抑制效果。阳性菌株通过PCR扩增筛选次级代谢产物生物合成基因PKS I、PKS II和NRPS。结果经形态学分析及16S rRNA基因序列对比后,从秋茄各组织部位中分离到50株内生细菌,隶属于21科27属。从秋茄叶子中分离到BGMRC2075菌株与已有细菌物种典型菌株的全长16S rRNA基因相似性为96.12%,提示其为潜在新种。37株细菌对甘蔗黑穗霉菌有抑制作用。其中,16株菌可能存在至少1种NRPS、PKS I和PKS II生物合成基因簇。结论秋茄中可培养细菌具有较高的物种多样性,蕴藏着潜在新物种资源,且富含抑菌活性的菌株。
[Abstract]:Objective to analyze the distribution characteristics and species diversity of endophytic bacteria of Kandelia candelia, a mangrove plant in Beihai Golden Bay, Guangxi, and to study the inhibitory activity of the crude extracts of bacteria metabolism on the black spike fungi of sugarcane. Methods 7 kinds of culture medium were used to isolate the culturable bacteria from the tissues of different parts of aubergine, and to establish the foundation for the development of new biological pesticides and biofilm preparations. The species diversity was analyzed by phylogenetic analysis of 16s rRNA gene sequence. The inhibitory effect of endophytic bacteria on sugarcane black panicularia was studied by Oxford Cup method. The secondary metabolite biosynthesis was screened by PCR amplification of positive strains. Results after morphological analysis and 16s rRNA gene sequence comparison, Fifty strains of endophytic bacteria were isolated from the tissues of aubergine. It belongs to 27 genera and 21 families. The similarity of 16s rRNA gene between BGMRC2075 strains isolated from leaves of autumn eggplant and typical strains of existing bacterial species is 96.12, which indicates that the 16s rRNA gene is a potential new species. 37 strains of bacteria have inhibitory effect on the black spike fungi of sugarcane. There may be at least one species of NRPSS-PKS I and PKS II biosynthesis gene clusters in 16 strains. Conclusion the culturable bacteria in aubergine have high species diversity. There are potential new species resources, and rich in bacteriostatic activity of strains.
【作者单位】：广西科学院广西海洋天然产物与组合生物合成化学重点实验室培育基地;广西医科大学广西生物医药协同创新中心;广西大学轻工与食品工程学院;广西中医药大学广西北部湾海洋中药应用技术与产品研发实验室;
【基金】：国家自然科学基金(No.81260480) 广西近海海洋环境科学重点实验室开放基金(No.GXKLHY13-06) 广西科学研究与技术开发计划(No.14123001-7) 广西自然科学基金(No.2014GXNSFAA118048,No.2015GXNSFBA139195) 广西高校科学技术研究(No.KY2015YB146) 广西重点实验室建设(No.15-140-31)
【分类号】：S435.661;S476.1

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