猪场废水厌氧消化液好氧处理过程酸化改进及菌群结构变化

发布时间：2018-02-26 15:16

本文关键词： 猪场废水厌氧消化液 SBR pH值 SrRna 厌氧氨氧化　出处：《中国沼气》2017年02期 　论文类型：期刊论文

【摘要】：采用序批式反应器(SBR)工艺直接处理猪场废水厌氧消化液,处理系统发生酸化,对COD和NH_4~+-N去除效率较低。针对这一问题,文章试验了两种改进方案,分别是接种厌氧氨氧化污泥(AN)和厌氧消化液中添加猪场废水原水(RW)。考察了不同改进方法对污染物的去除效果,并使用Miseq高通量测序技术分析了污泥中微生物多样性的变化情况。研究结果表明:与SBR直接处理猪场废水厌氧消化液相比,AN和RW组对COD去除率分别提高60.2%和102.6%,总无机氮(TIN)去除率分别提高了11.1%和73.3%,RW组的改进效果最明显。3个反应器中主要氨氧化细菌(AOB)是亚硝化单胞菌(Nitrosomonas),其对照(CG)组(7.9%)和AN组(4.2%)的相对丰度高于RW组(0.79%);RW组富集的氨氧化菌(AOB)相对丰度小于亚硝酸盐氧化菌(NOB);这些可解释CG组和AN组出现亚硝酸积累,而RW组没有出现亚硝酸盐积累。只有AN组出现厌氧氨氧化细菌的富集但相对丰度比较低(Candidatus_Brocadia=0.05%)。AN组厌氧氨氧化菌相对丰度低于高效的自养脱氮的需求。
[Abstract]:The anaerobic digestible liquid of pig farm wastewater was treated directly by sequencing batch reactor (SBR) process, the treatment system was acidified, and the removal efficiency of COD and NH4- N was low. In view of this problem, two improved schemes were tested in this paper. Inoculation of anaerobic ammonia oxidized sludge (ANN) and adding of raw water from pig farm wastewater to anaerobic digestible liquor were carried out respectively. The effects of different improved methods on the removal of pollutants were investigated. The variation of microbial diversity in sludge was analyzed by Miseq high-throughput sequencing technique. The results showed that the removal rate of COD was increased by 60.2% and 102.6 in an and RW groups, respectively, compared with the anaerobic digestible solution from pig farm wastewater treated directly by SBR. The removal efficiency of inorganic nitrogen tin increased by 11.1% and 73.3% respectively. The main ammonia-oxidizing bacteria AOB in the three reactors were Nitrosomonasone (7.9B) and an (4.2g).) the relative abundance of the three reactors was higher than the enrichment of RW 0.79m RW. The relative abundance of AOB was lower than that of nitrite oxidizing bacteria, which could explain the accumulation of nitrite in CG and an groups. However, nitrite accumulation was not found in group RW, but the concentration of anaerobic ammonia-oxidizing bacteria was found in group an, but the relative abundance was lower in group an than that in group an. The relative abundance of anaerobic ammonia-oxidizing bacteria in group an was lower than that of autotrophic denitrification in group an.
【作者单位】：农业部沼气科学研究所;农业部农村可再生能源开发利用重点实验室;
【基金】：国家自然科学基金(31572450) 国家生猪技术产业体系(CARS-36-10B)
【分类号】：X713

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