BIOLOG微平板技术检测农田土壤微生物群落结构的方法比较

发布时间：2018-02-27 13:33

本文关键词： BIOLOG-ECO微平板农田土壤微生物 AWCD 碳源代谢　出处：《华中农业大学学报》2017年03期 　论文类型：期刊论文

【摘要】：以采自湖北省荆州市和黄冈市的农田土壤作为供试土样,分别采用土壤样品冰浴后上清液100倍稀释、冰浴后悬浊液1 000倍稀释和离心去碳源3种方法,利用多功能酶标仪间隔12h检测D值,并计算AWCD(average well color development,每孔平均颜色变化)值来探究分析土壤肥力高、富含有机质、可利用碳源丰富的农田土壤微生物代谢功能的BIOLOG-ECO微平板技术适宜方法。结果表明,土样冰浴后上清液100倍稀释的方法不能有效去除土壤中可利用碳源,不能准确反映土壤微生物对碳源的利用程度。而冰浴后悬浊液1 000倍稀释、离心去碳源都能准确显示土壤微生物的活性变化,但是两者AWCD的标准差有极显著的差异,比较而言,离心去碳源的方法能更稳定地检测土壤微生物群落的代谢能力。研究结果为利用BIOLOG-ECO微平板技术检测富含有机质的农田土壤微生物代谢功能多样性提供了有价值的信息。
[Abstract]:The farmland soil collected from Jingzhou City and Huanggang City of Hubei Province was used as test soil samples. The soil samples were diluted by 100 times of supernatant after ice bath, 1000 times by suspensions after ice bath and 1 000 times by centrifugation of carbon source. Using a multifunctional enzyme marker to detect D value at 12h intervals and calculate the AWCD(average well color development (average color change per hole) to explore the analysis of high fertility and rich organic matter in the soil, The results showed that the 100 times dilution of soil supernatant after ice bath could not effectively remove the available carbon source in soil. It can not accurately reflect the utilization degree of soil microorganism to carbon source, but after ice bath, the suspensions of 1 000 times dilution, centrifugal decarbonization can accurately show the change of soil microbial activity, but the standard deviation of AWCD is very significant difference between them. The results of this study provide valuable information for the detection of microbial metabolic diversity in soil rich in organic matter by means of BIOLOG-ECO microplate technique.
【作者单位】：华中农业大学农业微生物学国家重点实验室;
【基金】：国家重点基础研究发展计划(“973”计划)(2015CB150504) 教育部长江学者和创新团队发展计划(IRT1247) 中央高校基本科研业务费专项(2662015PY016;2013PY111;2013PY136)
【分类号】：S154.3

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