衡阳紫色土丘陵坡地不同植被恢复阶段土壤微生物群落多样性的变化

发布时间：2018-02-28 06:27

本文关键词： Biolog-ECO 植被恢复土壤微生物群落多样性紫色土衡阳　出处：《林业科学》2016年08期 　论文类型：期刊论文

【摘要】：【目的】探讨衡阳紫色土丘陵坡地不同植被恢复阶段土壤微生物群落多样性的变化特征,明确土壤微生物群落多样性对植被恢复的响应规律,探明研究区域退化生态系统恢复机制,为构建该区域生态系统恢复技术体系提供理论依据。【方法】采用空间序列代替时间序列方法,在研究区域内选择立地基本相似的裸荒地、草本群落、灌木群落与乔木群落4种典型的植被类型表示恢复的4个阶段,运用Biolog-ECO微平板技术,对4个阶段的土壤微生物群落多样性进行研究。【结果】植被恢复后土壤微生物群落代谢活性显著升高(P0.05);相同土层不同恢复阶段代表土壤微生物活性和功能多样性的平均颜色变化率率大小顺序为乔木群落灌木群落草本群落y|荒地,相同恢复阶段不同土层的平均颜色变化率大小顺序为0~10 cm土层10~25 cm土层25~40 cm土层;4个恢复阶段土壤微生物群落的Shannon-Wiener物种丰富度指数、Shannon-Wiener均匀度指数、Simpson优势度指数和Mc Intosh指数以乔木群落最高(P0.05),草本群落和灌木群落其次(P0.05),草本群落最低(P0.05);各恢复阶段对不同C,N源利用强度的大小顺序为乔木群落灌木群落草本群落裸荒地(P0.05),不同土层对各C,N源利用强度的大小顺序为0~10 cm土层10~25 cm土层25~40 cm土层(P0.05);主成分分析表明,从31种C,N源类型提取的与C,N源利用相关的主成分1和主成分2分别能解释变量方差的37.24%和15.89%,在主成分分离中起主要贡献作用的C,N源为糖类、氨基酸类以及代谢中间产物和次生代谢物;相关分析表明,土壤含水量、土壤总有机碳、全氮、全磷和速效磷对土壤微生物群落多样性具有重要影响。【结论】植被恢复可使土壤微生物代谢功能增强,土壤微生物繁殖快、数量大,从而促进土壤微生物对土壤C,N源的利用强度。
[Abstract]:[objective] to study the variation characteristics of soil microbial community diversity in different vegetation restoration stages in Hengyang purple soil hilly land, and to clarify the response of soil microbial community diversity to vegetation restoration. To study the restoration mechanism of degraded ecosystem in the region, to provide the theoretical basis for constructing the technical system of ecosystem restoration in this region. [methods] using spatial sequence instead of time series method, the bare wasteland with similar sites was selected in the study area. The four typical vegetation types of herbaceous community, shrub community and Arbor community indicated four stages of restoration. Biolog-ECO microplate technique was used. The diversity of soil microbial communities in four stages was studied. [results] after vegetation restoration, the metabolic activities of soil microbial communities increased significantly (P 0.05), and different restoration stages of the same soil layer represented soil microbial activity and functional diversity. The order of the average rate of color change was as follows: tree community, shrub community, herbaceous community y wasteland, The order of average color change rate of different soil layers in the same restoration stage is 0 ~ 10cm soil layer 1025cm soil layer 250-40cm soil layer, the Shannon-Wiener species richness index of soil microbial community is Shannon-Wiener evenness index and the Simpson dominance index of soil microbial community in 4 restoration stages. MC Intosh index was the highest in Arbor community, P0.05 in herbaceous community and shrub community, and the lowest in herbaceous community. The order of N source utilization intensity of each layer is 0 ~ 10cm soil layer (1025cm) and 40cm soil layer (P0.05N), and the principal component analysis shows that, The principal component 1 and principal component 2, which are related to the utilization of Con N source, can explain 37.24% and 15.89 variance of variables, respectively, and the Con N source, which plays a major role in the separation of principal components, is sugar. Amino acids and metabolic intermediates and secondary metabolites; correlation analysis showed that soil water content, total organic carbon, total nitrogen, Total phosphorus and available phosphorus have an important effect on the diversity of soil microbial communities. [conclusion] vegetation restoration can enhance the metabolic function of soil microbes, and soil microbes reproduce rapidly and in large quantities. Therefore, the utilization intensity of soil Con N source was promoted by soil microorganism.
【作者单位】：湖南环境生物职业技术学院园林学院;湖南农业大学生物科学技术学院;
【基金】：湖南省科技厅项目(S2006N332) 湖南省教育厅科学研究项目(12C1057) 湖南省林业科技创新计划项目(XLK201341) 湖南省衡阳市农业科技支撑计划项目(2014KN27) 湖南省普通高校优秀青年骨干教师培养对象资助项目湖南环境生物职业技术学院南岳学者基金项目
【分类号】：S154.3

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