庞泉沟自然保护区华北落叶松与桦树林土壤微生物群落结构

发布时间：2018-03-11 04:11

本文选题：森林土壤微生物　切入点：群落多样性　出处：《应用与环境生物学报》2017年03期 　论文类型：期刊论文

【摘要】：落叶针叶林和落叶阔叶林是华北地区主要的森林类型,其地下生态系统在驱动生物地球化学循环过程中发挥重要作用.运用Illumina高通量测序技术分析庞泉沟自然保护区中海拔桦树(Betula platyphylla)林和华北落叶松(Larix principis-rupprechtii)林以及高海拔华北落叶松林的土壤微生物群落结构,同时对土壤过氧化氢酶、脲酶、蔗糖酶活性及土壤理化性质进行测定,分析各因子的变化规律及其之间的相关性.结果显示:1)3个样地土壤脲酶、蔗糖酶活性与全碳、全氮、全硫、碳氮比呈极显著正相关;脲酶活性与p H显著负相关;过氧化氢酶活性与土壤理化性质均无显著相关性;同时3种酶的活性与细菌和真菌特定类群的丰度密切相关.2)样地间土壤细菌群落结构具有一定差异,而真菌群落结构的差异较大,土壤理化性质对微生物群落的结构具有较大的影响.真菌群落中的煤炱目(Capnodiales)、蜡壳耳目(Sebacinales)、路霉目(Lulworthiales)、锈革孔菌目(Hymenochaetales)的丰度与土壤全碳、全氮、全硫、碳氮比、含水率显著相关.3)中海拔桦树林土壤细菌群落多样性和丰度高于华北落叶松林,真菌群落的丰度与之相反;高海拔落叶松林细菌群落多样性较低,而丰度较高,真菌群落则是丰度较低,多样性在高海拔落叶松林中最高,在桦树林中较低.综上,植被类型、土壤理化性质和微生物群落结构三者相互影响,因此可通过改变林下土壤微生物环境,制定出不同的育林措施,进而影响土壤生态系统的碳、氮、硫等循环进程,提高土壤肥力.
[Abstract]:Deciduous coniferous forest and deciduous broad-leaved forest are the main forest types in North China. The underground ecosystem plays an important role in driving biogeochemical cycle. Analysis of Betula platyphylla) forest and Larix principis-rupprechtii forest in Pang Quangou Nature Reserve and high altitude using Illumina high-throughput sequencing technique. Soil microbial Community structure of Larch Forest in North China, The soil catalase, urease, sucrase activity and soil physicochemical properties were determined, and the change rules of the factors and their correlation were analyzed. The results showed that the soil urease, sucrase activity and total carbon, total nitrogen were correlated with the soil catalase, urease and sucrase activity. The ratio of total sulfur to carbon to nitrogen was significantly positive, urease activity was negatively correlated with pH, and catalase activity had no significant correlation with soil physical and chemical properties. At the same time, the activities of the three enzymes were closely related to the abundance of bacteria and specific groups of fungi. 2) there were some differences in soil bacterial community structure among the plots, but there was a great difference in the structure of fungi community. The physical and chemical properties of soil have great influence on the structure of microbial community. The abundance and soil total carbon, total nitrogen, total sulfur, carbon and nitrogen ratio of the order Capnodialesa, Sebacinalesus, Lulworthialesus, Hymenochaetaleses in the fungal community, and the ratio of total carbon, total nitrogen, total sulfur, carbon to nitrogen, total nitrogen, total nitrogen, total sulfur, carbon and nitrogen, and the ratio of total carbon, total nitrogen, total sulfur, carbon and nitrogen to soil total carbon, total nitrogen, total sulfur, carbon and nitrogen ratio. Soil bacterial community diversity and abundance in birch forest were higher than that in North China larch forest, but the abundance of fungal community was opposite, but the diversity of bacterial community was lower and abundance was higher in larch forest at high altitude. The diversity of fungi community was the highest in the larch forest at high altitude and the lowest in the birch forest. In summary, the vegetation types, soil physical and chemical properties and microbial community structure interacted with each other. Therefore, by changing the soil microbial environment under the forest, different forestry measures can be worked out to influence the circulation of carbon, nitrogen and sulfur in the soil ecosystem and to improve soil fertility.
【作者单位】：山西大学黄土高原研究所;山西财经大学环境经济学院;山西文峪河国家级湿地公园管理局;
【基金】：国家自然科学基金项目(31540054) 山西省回国留学人员科研资助项目(2012-059) 山西省科技攻关项目(20150313001-3)~~
【分类号】：S714.3

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